Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015295153> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2015295153 abstract "Alternative splicing of exon 2, 3 and 10 of the tau gene results in six isoforms of tau in the adult CNS. Exon 10 inclusion results in tau isoforms containing four microtubule binding repeats (4R-tau) and exclusion results in three repeats (3R-tau). The tauopathies including PSP, FTDP-17, CBD and Pick's disease are linked to differential expression of 3R- and 4R-tau. The aim of the study was to measure the levels of 3R and 4R tau protein in PSP and control brains using a sandwich ELISA and to quantify tau mRNA isoforms by real-time PCR. Protein and mRNA levels of 3R- and 4R- tau in frontal cortex, caudate nucleus, globus pallidus (affected regions) and occipital lobe (unaffected regions) from confirmed PSP cases and control brains were measured using sandwich ELISAs and real-time PCR respectively. Monoclonal antibodies: RD3 or RD4 were used as coating antigens in the ELISAs and affinity purified- anti-TAU peptide HRP conjugate as the detector antibody. Total RNA from selected regions was extracted and real-time quantitative PCR was carried out using Sybr Green. There is no statistically significant difference in tau protein and mRNA levels between an affected area (frontal cortex) and an unaffected area (occipital lobe) of control brains. A significant increase (1.6-fold) in 4R-tau protein levels (ng/mg of total protein) as well as a trend for increased 4R- tau mRNA were observed in the PSP frontal cortex compared to control brains. However, there are no differences in 3R-tau levels in the frontal cortices between PSP and control brains. Furthermore, we observed no differences in both 3R- and 4R-tau levels in the occipital lobe (area spared from tau pathology) of PSP and control brains. In summary, we have used a recently developed sandwich ELISA for direct quantification of 3R- and 4R- tau in pathological samples. We have also used real-time PCR to measure mRNA expression of these tau isoforms. In this study, we have shown that abnormalities in protein levels as well as mRNA expression of 3R- and 4R- tau are both involved in the pathogenesis of PSP." @default.
- W2015295153 created "2016-06-24" @default.
- W2015295153 creator A5040684208 @default.
- W2015295153 creator A5057145376 @default.
- W2015295153 creator A5058433445 @default.
- W2015295153 creator A5087073870 @default.
- W2015295153 date "2008-07-01" @default.
- W2015295153 modified "2023-09-27" @default.
- W2015295153 title "P2-174: Expression of three-repeat and four-repeat tau isoforms and mRNA levels in control and progressive supranuclear palsy brains" @default.
- W2015295153 doi "https://doi.org/10.1016/j.jalz.2008.05.1248" @default.
- W2015295153 hasPublicationYear "2008" @default.
- W2015295153 type Work @default.
- W2015295153 sameAs 2015295153 @default.
- W2015295153 citedByCount "0" @default.
- W2015295153 crossrefType "journal-article" @default.
- W2015295153 hasAuthorship W2015295153A5040684208 @default.
- W2015295153 hasAuthorship W2015295153A5057145376 @default.
- W2015295153 hasAuthorship W2015295153A5058433445 @default.
- W2015295153 hasAuthorship W2015295153A5087073870 @default.
- W2015295153 hasBestOaLocation W20152951531 @default.
- W2015295153 hasConcept C104317684 @default.
- W2015295153 hasConcept C105580179 @default.
- W2015295153 hasConcept C126322002 @default.
- W2015295153 hasConcept C134018914 @default.
- W2015295153 hasConcept C153911025 @default.
- W2015295153 hasConcept C169760540 @default.
- W2015295153 hasConcept C194583182 @default.
- W2015295153 hasConcept C2776477761 @default.
- W2015295153 hasConcept C2776925932 @default.
- W2015295153 hasConcept C2777739294 @default.
- W2015295153 hasConcept C2778187257 @default.
- W2015295153 hasConcept C2778670691 @default.
- W2015295153 hasConcept C2779134260 @default.
- W2015295153 hasConcept C2780278869 @default.
- W2015295153 hasConcept C2781172350 @default.
- W2015295153 hasConcept C2993858690 @default.
- W2015295153 hasConcept C36823959 @default.
- W2015295153 hasConcept C502032728 @default.
- W2015295153 hasConcept C529278444 @default.
- W2015295153 hasConcept C53345823 @default.
- W2015295153 hasConcept C54355233 @default.
- W2015295153 hasConcept C54458228 @default.
- W2015295153 hasConcept C67705224 @default.
- W2015295153 hasConcept C71924100 @default.
- W2015295153 hasConcept C86803240 @default.
- W2015295153 hasConceptScore W2015295153C104317684 @default.
- W2015295153 hasConceptScore W2015295153C105580179 @default.
- W2015295153 hasConceptScore W2015295153C126322002 @default.
- W2015295153 hasConceptScore W2015295153C134018914 @default.
- W2015295153 hasConceptScore W2015295153C153911025 @default.
- W2015295153 hasConceptScore W2015295153C169760540 @default.
- W2015295153 hasConceptScore W2015295153C194583182 @default.
- W2015295153 hasConceptScore W2015295153C2776477761 @default.
- W2015295153 hasConceptScore W2015295153C2776925932 @default.
- W2015295153 hasConceptScore W2015295153C2777739294 @default.
- W2015295153 hasConceptScore W2015295153C2778187257 @default.
- W2015295153 hasConceptScore W2015295153C2778670691 @default.
- W2015295153 hasConceptScore W2015295153C2779134260 @default.
- W2015295153 hasConceptScore W2015295153C2780278869 @default.
- W2015295153 hasConceptScore W2015295153C2781172350 @default.
- W2015295153 hasConceptScore W2015295153C2993858690 @default.
- W2015295153 hasConceptScore W2015295153C36823959 @default.
- W2015295153 hasConceptScore W2015295153C502032728 @default.
- W2015295153 hasConceptScore W2015295153C529278444 @default.
- W2015295153 hasConceptScore W2015295153C53345823 @default.
- W2015295153 hasConceptScore W2015295153C54355233 @default.
- W2015295153 hasConceptScore W2015295153C54458228 @default.
- W2015295153 hasConceptScore W2015295153C67705224 @default.
- W2015295153 hasConceptScore W2015295153C71924100 @default.
- W2015295153 hasConceptScore W2015295153C86803240 @default.
- W2015295153 hasIssue "4S_Part_12" @default.
- W2015295153 hasLocation W20152951531 @default.
- W2015295153 hasOpenAccess W2015295153 @default.
- W2015295153 hasPrimaryLocation W20152951531 @default.
- W2015295153 hasRelatedWork W1958597028 @default.
- W2015295153 hasRelatedWork W1987950120 @default.
- W2015295153 hasRelatedWork W2015246275 @default.
- W2015295153 hasRelatedWork W2083847629 @default.
- W2015295153 hasRelatedWork W2092836640 @default.
- W2015295153 hasRelatedWork W2158816574 @default.
- W2015295153 hasRelatedWork W2745723420 @default.
- W2015295153 hasRelatedWork W3163675851 @default.
- W2015295153 hasRelatedWork W3200077090 @default.
- W2015295153 hasRelatedWork W4220794037 @default.
- W2015295153 hasVolume "4" @default.
- W2015295153 isParatext "false" @default.
- W2015295153 isRetracted "false" @default.
- W2015295153 magId "2015295153" @default.
- W2015295153 workType "article" @default.