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• W2015299128 abstract "After cardiovascular intervention, smooth muscle cells (SMC) are directly exposed to blood flow and thus their behavior might be affected by fluid hemodynamic forces. The aim of this study was to determine the effect of fluid shear stress on the growth rate of SMC. Human aortic smooth muscle cells (hASMC) were seeded on fibronectin-coated glass slides and were exposed to different levels of shear stress using parallel plate flow chambers. After 24 h, cell numbers in the stationary and sheared cultures were measured by a Coulter counter. Results demonstrated that increasing shear stress significantly reduces the proliferation rate of hASMC (P < 0.05). Comparable lactate dehydrogenase levels in the media of stationary and flow cultures provided evidence that the reduction of cell number was not due to cell injury. Proliferating cell nuclear antigen (PCNA) immunofluorescence studies indicated that the cell cultures were not growth arrested 24 h after exposure to shear stress, and that the differences in PCNA staining between stationary control and flow cultures were comparable to the cell counts. © 1996 John Wiley & Sons, Inc." @default.
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• W2015299128 date "2000-03-26" @default.
• W2015299128 modified "2023-09-26" @default.
• W2015299128 title "Effects of shear stress on the growth kinetics of human aortic smooth muscle cells in vitro" @default.
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• W2015299128 doi "https://doi.org/10.1002/(sici)1097-0290(19960605)50:5<555::aid-bit10>3.0.co;2-i" @default.
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