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• W2015303203 startingPage "e12315" @default.
• W2015303203 abstract "The Math5 (Atoh7) gene is transiently expressed during retinogenesis by progenitors exiting mitosis, and is essential for ganglion cell (RGC) development. Math5 contains a single exon, and its 1.7 kb mRNA encodes a 149-aa polypeptide. Mouse Math5 mutants have essentially no RGCs or optic nerves. Given the importance of this gene in retinal development, we thoroughly investigated the possibility of Math5 mRNA splicing by Northern blot, 3'RACE, RNase protection assays, and RT-PCR, using RNAs extracted from embryonic eyes and adult cerebellum, or transcribed in vitro from cDNA clones. Because Math5 mRNA contains an elevated G+C content, we used graded concentrations of betaine, an isostabilizing agent that disrupts secondary structure. Although approximately 10% of cerebellar Math5 RNAs are spliced, truncating the polypeptide, our results show few, if any, spliced Math5 transcripts exist in the developing retina (<1%). Rare deleted cDNAs do arise via RT-mediated RNA template switching in vitro, and are selectively amplified during PCR. These data differ starkly from a recent study (Kanadia and Cepko 2010), which concluded that the vast majority of Math5 and other bHLH transcripts are spliced to generate noncoding RNAs. Our findings clarify the architecture of the Math5 gene and its mechanism of action. These results have implications for all members of the bHLH gene family, for any gene that is alternatively spliced, and for the interpretation of all RT-PCR experiments." @default.
• W2015303203 created "2016-06-24" @default.
• W2015303203 creator A5024086191 @default.
• W2015303203 creator A5069325928 @default.
• W2015303203 creator A5090177774 @default.
• W2015303203 date "2010-08-24" @default.
• W2015303203 modified "2023-10-14" @default.
• W2015303203 title "A Critical Analysis of Atoh7 (Math5) mRNA Splicing in the Developing Mouse Retina" @default.
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• W2015303203 doi "https://doi.org/10.1371/journal.pone.0012315" @default.
• W2015303203 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2927423" @default.
• W2015303203 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20808762" @default.
• W2015303203 hasPublicationYear "2010" @default.
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