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- W2015307990 abstract "Upon reaction of luciferase-FMNH2 with oxygen a complex series of absorbance changes occur, leading to the formation of a stable (t12 about 35 min at 2°) dihydroflavin peroxyluciferase intermediate. Observed at 380, 445, or 600 nm, there is first a rapid absorbance increase which is oxygen-concentration dependent (k ⋍ 106 M−1s−1. Following this there are two oxygen independent steps, first a slow absorbance decrease (k = 4.3 s−1) and then an even slower increase (k = 0.55 s−1). The dihydroflavin peroxide is not expected to have absorption at 600 nm and is thus postulated to be in equilibrium with some flavin species which does absorb in the red." @default.
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- W2015307990 title "Oxygen dependent and independent steps in luciferase-FMNH 2 oxidation" @default.
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- W2015307990 doi "https://doi.org/10.1016/0006-291x(78)90881-1" @default.
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