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• W2015332008 abstract "Plasma-based methods have recently emerged as a technique for augmenting plasmid DNA delivery to skin. This delivery modality relies on the deposition of ionized gas molecules on to targeted cells or tissue to establish an electric field. It is hypothesized that this electric field results in the dielectric breakdown of cell membranes, making cells permeable to exogenous molecules. This in vivo investigation sought to optimize the intradermal delivery of a luciferase expressing plasmid DNA by modulating the total exposure to the plasma source and the plasmid DNA dose. Varying the plasma exposure time from 2, 5, 10, and 20 min allowed the conditions resulting in the highest expression of luciferase to be found. These conditions correlated to the 10 minute exposure time for a plasma derived from either + 8 kV or − 8 kV, when the generator was operated 3 cm from the epidermal tissue surface with a helium flow rate of 15 L/min. Exposing the injected flank skin for 10 min resulted in a rise of 37.3-fold for a plasma created with + 8 kV and 27.1-fold for a plasma created with − 8 kV. When using this treatment time with 50, 100, or 200 μg of a luciferase expressing plasmid, it was found that 100 μg resulted in the highest peak luminescence." @default.
• W2015332008 created "2016-06-24" @default.
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• W2015332008 date "2015-06-01" @default.
• W2015332008 modified "2023-09-30" @default.
• W2015332008 title "Optimization of a plasma facilitated DNA delivery method" @default.
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• W2015332008 doi "https://doi.org/10.1016/j.bioelechem.2014.09.003" @default.
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