FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015334002> ?p ?o ?g. }

• W2015334002 endingPage "295" @default.
• W2015334002 startingPage "281" @default.
• W2015334002 abstract "A separation chamber of 90 ml effective volume is described, that incorporates a sector shape (to prevent wall sedimentation) and anti-vortex lamellae (to prevent swirling upon acceleration and deceleration) as well as a flow-diverter for undisturbed layering and fractionation. The chamber is run in an ordinary table centrifuge and possesses a resolving power for equilibrium density centrifugation that seems unsurpassed. Peripheral human blood cells were separated in a Percoll gradient yielding in 1 single run the concurrent isolation of 99% pure monocytes, 99% pure lymphocytes and 99% pure granulocytes. Recoveries varied from 94% to 102%. By modifying the shape of the density gradient, either 99% of all monocytes collected were more than 92.5% pure and at the same time separation of small versus large lymphocytes was obtained, or substantial purification of neutrophils and eosinophils was achieved. The potential for simultaneous separation of monocytes, B, T cells and granulocytes was established. Good separations were obtained with buffy coats from 1 U of blood. The chamber also functioned well in the standard Böyum method for mononuclear cell preparation. In addition, a method for the rapid removal of erythrocytes (within 9 min) at unit gravity is described with nearly quantitative yield of white cells." @default.
• W2015334002 created "2016-06-24" @default.
• W2015334002 creator A5001551450 @default.
• W2015334002 creator A5075250909 @default.
• W2015334002 date "1984-04-01" @default.
• W2015334002 modified "2023-10-17" @default.
• W2015334002 title "A separation chamber to sort cells and cell organelles by weak physical forces. V. A sector-shaped chamber and its application to the separation of peripheral blood cells" @default.
• W2015334002 cites W123315612 @default.
• W2015334002 cites W1502694991 @default.
• W2015334002 cites W1508998225 @default.
• W2015334002 cites W1584531376 @default.
• W2015334002 cites W1980535421 @default.
• W2015334002 cites W2008184154 @default.
• W2015334002 cites W2018081460 @default.
• W2015334002 cites W2018537561 @default.
• W2015334002 cites W2026335497 @default.
• W2015334002 cites W2030764175 @default.
• W2015334002 cites W2034079730 @default.
• W2015334002 cites W2034578584 @default.
• W2015334002 cites W2035309658 @default.
• W2015334002 cites W2036879112 @default.
• W2015334002 cites W2038802741 @default.
• W2015334002 cites W2042698728 @default.
• W2015334002 cites W2052235307 @default.
• W2015334002 cites W2053704219 @default.
• W2015334002 cites W2059161060 @default.
• W2015334002 cites W2061568689 @default.
• W2015334002 cites W2073087079 @default.
• W2015334002 cites W2085506328 @default.
• W2015334002 cites W2089567287 @default.
• W2015334002 cites W2091470079 @default.
• W2015334002 cites W2094640034 @default.
• W2015334002 cites W2157437695 @default.
• W2015334002 cites W2165880962 @default.
• W2015334002 cites W2257840380 @default.
• W2015334002 cites W268102908 @default.
• W2015334002 cites W302589014 @default.
• W2015334002 cites W74241357 @default.
• W2015334002 cites W1538274455 @default.
• W2015334002 doi "https://doi.org/10.1016/0022-1759(84)90326-0" @default.
• W2015334002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6325544" @default.
• W2015334002 hasPublicationYear "1984" @default.
• W2015334002 type Work @default.
• W2015334002 sameAs 2015334002 @default.
• W2015334002 citedByCount "5" @default.
• W2015334002 crossrefType "journal-article" @default.
• W2015334002 hasAuthorship W2015334002A5001551450 @default.
• W2015334002 hasAuthorship W2015334002A5075250909 @default.
• W2015334002 hasConcept C121332964 @default.
• W2015334002 hasConcept C12554922 @default.
• W2015334002 hasConcept C137061746 @default.
• W2015334002 hasConcept C150867306 @default.
• W2015334002 hasConcept C185592680 @default.
• W2015334002 hasConcept C192562407 @default.
• W2015334002 hasConcept C202751555 @default.
• W2015334002 hasConcept C203014093 @default.
• W2015334002 hasConcept C2778285947 @default.
• W2015334002 hasConcept C2778892434 @default.
• W2015334002 hasConcept C43617362 @default.
• W2015334002 hasConcept C48679268 @default.
• W2015334002 hasConcept C55493867 @default.
• W2015334002 hasConcept C62520636 @default.
• W2015334002 hasConcept C7623868 @default.
• W2015334002 hasConcept C86803240 @default.
• W2015334002 hasConceptScore W2015334002C121332964 @default.
• W2015334002 hasConceptScore W2015334002C12554922 @default.
• W2015334002 hasConceptScore W2015334002C137061746 @default.
• W2015334002 hasConceptScore W2015334002C150867306 @default.
• W2015334002 hasConceptScore W2015334002C185592680 @default.
• W2015334002 hasConceptScore W2015334002C192562407 @default.
• W2015334002 hasConceptScore W2015334002C202751555 @default.
• W2015334002 hasConceptScore W2015334002C203014093 @default.
• W2015334002 hasConceptScore W2015334002C2778285947 @default.
• W2015334002 hasConceptScore W2015334002C2778892434 @default.
• W2015334002 hasConceptScore W2015334002C43617362 @default.
• W2015334002 hasConceptScore W2015334002C48679268 @default.
• W2015334002 hasConceptScore W2015334002C55493867 @default.
• W2015334002 hasConceptScore W2015334002C62520636 @default.
• W2015334002 hasConceptScore W2015334002C7623868 @default.
• W2015334002 hasConceptScore W2015334002C86803240 @default.
• W2015334002 hasIssue "2" @default.
• W2015334002 hasLocation W20153340021 @default.
• W2015334002 hasLocation W20153340022 @default.
• W2015334002 hasOpenAccess W2015334002 @default.
• W2015334002 hasPrimaryLocation W20153340021 @default.
• W2015334002 hasRelatedWork W1998156145 @default.
• W2015334002 hasRelatedWork W2009648089 @default.
• W2015334002 hasRelatedWork W2015334002 @default.
• W2015334002 hasRelatedWork W2017458083 @default.
• W2015334002 hasRelatedWork W2094640034 @default.
• W2015334002 hasRelatedWork W2111021139 @default.
• W2015334002 hasRelatedWork W2229848870 @default.
• W2015334002 hasRelatedWork W2438523498 @default.
• W2015334002 hasRelatedWork W3149207744 @default.
• W2015334002 hasRelatedWork W91741394 @default.
• W2015334002 hasVolume "69" @default.
• W2015334002 isParatext "false" @default.
• W2015334002 isRetracted "false" @default.

• next