FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015363531> ?p ?o ?g. }

• W2015363531 endingPage "67" @default.
• W2015363531 startingPage "59" @default.
• W2015363531 abstract "Objectives Malignant cells of mature B-cell origin show tumor-specific clonal immunoglobulin gene (IG) rearrangements, including V(D)J recombinations, nucleotide mutations, or translocations. Rapid molecular cloning of the breakpoint sequence by long-distance inverse PCR (LDI-PCR) has so far been applied to rearrangements targeted to IGH joining, IGH switch, and IGκ regions. We tended to apply LDI-PCR method for cloning of IGλ rearrangements. Methods To identify which IGλ isotype segment was rearranged, we performed Southern blot analysis using isotype-specific probes. We set inverse primers on the telomeric side of each joining region and amplified rearranged bands detected by Southern blot analysis as corresponding PCR products. Results All germline IGλ segments were successfully amplified as expected PCR products. We determined breakpoint sequences of five chromosome translocations involving IGλ locus: three novel t(8;22)(q24;q11), one known t(3;22)(q27;q11), and one partially known t(11;22)(q13;q11). Two of the three t(8;22)(q24;q11) were involved in Jλ with a recombination signal sequence and one of three in the first exon of IGLL5, which lies upstream of Jλ1. Three 8q24 breakpoints were widespread at 132, 260 and 366 kb downstream of MYC locus. The t(3;22)(q27;q11) showed a juxtaposition of Jλ2 and the first intron of BCL6, as previously reported. In t(11;22)(q13;q11), 3′UTR of cyclin D1 fused to the constant region of λ7 with nucleotide mutations. We also amplified four Vλ/Jλ recombination sequences. Conclusion Our method is a useful tool for molecular analysis of genetic events in IGλ." @default.
• W2015363531 created "2016-06-24" @default.
• W2015363531 creator A5006958303 @default.
• W2015363531 creator A5017608352 @default.
• W2015363531 creator A5023065633 @default.
• W2015363531 creator A5029993402 @default.
• W2015363531 creator A5038371406 @default.
• W2015363531 creator A5048425568 @default.
• W2015363531 creator A5062508248 @default.
• W2015363531 creator A5081531978 @default.
• W2015363531 creator A5087064410 @default.
• W2015363531 date "2012-12-04" @default.
• W2015363531 modified "2023-10-17" @default.
• W2015363531 title "Molecular cloning of<i>IGλ</i>rearrangements using long-distance inverse PCR (LDI-PCR)" @default.
• W2015363531 cites W1483687177 @default.
• W2015363531 cites W1568951543 @default.
• W2015363531 cites W1576401187 @default.
• W2015363531 cites W1777379465 @default.
• W2015363531 cites W2006729924 @default.
• W2015363531 cites W2011330486 @default.
• W2015363531 cites W2013898255 @default.
• W2015363531 cites W2036166452 @default.
• W2015363531 cites W2055043387 @default.
• W2015363531 cites W2055597910 @default.
• W2015363531 cites W2064223130 @default.
• W2015363531 cites W2065138021 @default.
• W2015363531 cites W206592526 @default.
• W2015363531 cites W2068371335 @default.
• W2015363531 cites W2070484767 @default.
• W2015363531 cites W2084760580 @default.
• W2015363531 cites W2086538453 @default.
• W2015363531 cites W2096313716 @default.
• W2015363531 cites W2127134141 @default.
• W2015363531 cites W2127398547 @default.
• W2015363531 cites W2127640118 @default.
• W2015363531 cites W2150938787 @default.
• W2015363531 cites W31386547 @default.
• W2015363531 cites W36484103 @default.
• W2015363531 cites W4248493569 @default.
• W2015363531 doi "https://doi.org/10.1111/ejh.12037" @default.
• W2015363531 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23113889" @default.
• W2015363531 hasPublicationYear "2012" @default.
• W2015363531 type Work @default.
• W2015363531 sameAs 2015363531 @default.
• W2015363531 citedByCount "6" @default.
• W2015363531 countsByYear W20153635312013 @default.
• W2015363531 countsByYear W20153635312014 @default.
• W2015363531 countsByYear W20153635312015 @default.
• W2015363531 countsByYear W20153635312017 @default.
• W2015363531 countsByYear W20153635312021 @default.
• W2015363531 crossrefType "journal-article" @default.
• W2015363531 hasAuthorship W2015363531A5006958303 @default.
• W2015363531 hasAuthorship W2015363531A5017608352 @default.
• W2015363531 hasAuthorship W2015363531A5023065633 @default.
• W2015363531 hasAuthorship W2015363531A5029993402 @default.
• W2015363531 hasAuthorship W2015363531A5038371406 @default.
• W2015363531 hasAuthorship W2015363531A5048425568 @default.
• W2015363531 hasAuthorship W2015363531A5062508248 @default.
• W2015363531 hasAuthorship W2015363531A5081531978 @default.
• W2015363531 hasAuthorship W2015363531A5087064410 @default.
• W2015363531 hasConcept C104317684 @default.
• W2015363531 hasConcept C113646796 @default.
• W2015363531 hasConcept C133307689 @default.
• W2015363531 hasConcept C138626823 @default.
• W2015363531 hasConcept C153911025 @default.
• W2015363531 hasConcept C156695909 @default.
• W2015363531 hasConcept C157402786 @default.
• W2015363531 hasConcept C157431215 @default.
• W2015363531 hasConcept C20682886 @default.
• W2015363531 hasConcept C206936463 @default.
• W2015363531 hasConcept C49105822 @default.
• W2015363531 hasConcept C54355233 @default.
• W2015363531 hasConcept C84597430 @default.
• W2015363531 hasConcept C86803240 @default.
• W2015363531 hasConceptScore W2015363531C104317684 @default.
• W2015363531 hasConceptScore W2015363531C113646796 @default.
• W2015363531 hasConceptScore W2015363531C133307689 @default.
• W2015363531 hasConceptScore W2015363531C138626823 @default.
• W2015363531 hasConceptScore W2015363531C153911025 @default.
• W2015363531 hasConceptScore W2015363531C156695909 @default.
• W2015363531 hasConceptScore W2015363531C157402786 @default.
• W2015363531 hasConceptScore W2015363531C157431215 @default.
• W2015363531 hasConceptScore W2015363531C20682886 @default.
• W2015363531 hasConceptScore W2015363531C206936463 @default.
• W2015363531 hasConceptScore W2015363531C49105822 @default.
• W2015363531 hasConceptScore W2015363531C54355233 @default.
• W2015363531 hasConceptScore W2015363531C84597430 @default.
• W2015363531 hasConceptScore W2015363531C86803240 @default.
• W2015363531 hasIssue "1" @default.
• W2015363531 hasLocation W20153635311 @default.
• W2015363531 hasLocation W20153635312 @default.
• W2015363531 hasOpenAccess W2015363531 @default.
• W2015363531 hasPrimaryLocation W20153635311 @default.
• W2015363531 hasRelatedWork W1483687177 @default.
• W2015363531 hasRelatedWork W1605698134 @default.
• W2015363531 hasRelatedWork W2021724827 @default.
• W2015363531 hasRelatedWork W2028087115 @default.
• W2015363531 hasRelatedWork W2040427754 @default.

• next