Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015382308> ?p ?o ?g. }
- W2015382308 endingPage "675" @default.
- W2015382308 startingPage "667" @default.
- W2015382308 abstract "Integration host factor (IHF), a nucleoid-associated protein in bacterial cells, is implicated in a number of chromosomal functions including DNA compaction. IHF binds to all duplex DNA with micromolar affinity and at sequence-specific sites with much higher affinity. IHF is known to induce sharp bends in the helical axis of DNA in both modes of binding, but the role of IHF in controlling DNA condensation within bacterial cells has remained undetermined. Here we demonstrate that IHF influences the morphology of DNA condensed by polyamines in vitro. In the absence of IHF, spermidine and spermine condense DNA primarily into toroidal structures, whereas in the presence of IHF, polyamines condense DNA primarily into rodlike structures. Computer simulations of DNA condensation in the absence and presence of IHF binding lend support to our model in which DNA bending proteins, such as IHF and HU, promote the condensation of DNA into rodlike structures by providing the free energy necessary to bend DNA at the ends of linear bundles of condensed DNA. We propose that a common function of IHF and HU in bacterial cells is to facilitate DNA organization in the nucleoid by the introduction of sharp bends in chromosomal DNA." @default.
- W2015382308 created "2016-06-24" @default.
- W2015382308 creator A5019974816 @default.
- W2015382308 creator A5026000559 @default.
- W2015382308 creator A5047716120 @default.
- W2015382308 creator A5060856297 @default.
- W2015382308 creator A5067399385 @default.
- W2015382308 creator A5088307660 @default.
- W2015382308 creator A5091747812 @default.
- W2015382308 date "2009-01-08" @default.
- W2015382308 modified "2023-09-26" @default.
- W2015382308 title "Integration Host Factor (IHF) Dictates the Structure of Polyamine-DNA Condensates: Implications for the Role of IHF in the Compaction of Bacterial Chromatin" @default.
- W2015382308 cites W1523985712 @default.
- W2015382308 cites W1533307088 @default.
- W2015382308 cites W1544991224 @default.
- W2015382308 cites W1803410052 @default.
- W2015382308 cites W1858539678 @default.
- W2015382308 cites W1965409637 @default.
- W2015382308 cites W1967161466 @default.
- W2015382308 cites W1967174711 @default.
- W2015382308 cites W1974930209 @default.
- W2015382308 cites W1977422953 @default.
- W2015382308 cites W1984360565 @default.
- W2015382308 cites W1991011918 @default.
- W2015382308 cites W1992087077 @default.
- W2015382308 cites W1993724696 @default.
- W2015382308 cites W1996614887 @default.
- W2015382308 cites W2000558089 @default.
- W2015382308 cites W2001118497 @default.
- W2015382308 cites W2003925078 @default.
- W2015382308 cites W2004791849 @default.
- W2015382308 cites W2008911030 @default.
- W2015382308 cites W2011263484 @default.
- W2015382308 cites W2016428273 @default.
- W2015382308 cites W2020002410 @default.
- W2015382308 cites W2032359606 @default.
- W2015382308 cites W2032767707 @default.
- W2015382308 cites W2035266068 @default.
- W2015382308 cites W2036098864 @default.
- W2015382308 cites W2040154659 @default.
- W2015382308 cites W2042980405 @default.
- W2015382308 cites W2043106598 @default.
- W2015382308 cites W2045824221 @default.
- W2015382308 cites W2046240935 @default.
- W2015382308 cites W2049083521 @default.
- W2015382308 cites W2052221476 @default.
- W2015382308 cites W2052514587 @default.
- W2015382308 cites W2052948676 @default.
- W2015382308 cites W2053328000 @default.
- W2015382308 cites W2059541577 @default.
- W2015382308 cites W2060417297 @default.
- W2015382308 cites W2062201957 @default.
- W2015382308 cites W2068370729 @default.
- W2015382308 cites W2072576748 @default.
- W2015382308 cites W2075596893 @default.
- W2015382308 cites W2082927796 @default.
- W2015382308 cites W2083278118 @default.
- W2015382308 cites W2085655662 @default.
- W2015382308 cites W2086174686 @default.
- W2015382308 cites W2090099066 @default.
- W2015382308 cites W2090631246 @default.
- W2015382308 cites W2092563618 @default.
- W2015382308 cites W2099105015 @default.
- W2015382308 cites W2102937661 @default.
- W2015382308 cites W2105976857 @default.
- W2015382308 cites W2109339776 @default.
- W2015382308 cites W2116395964 @default.
- W2015382308 cites W2119109353 @default.
- W2015382308 cites W2127552033 @default.
- W2015382308 cites W2132664565 @default.
- W2015382308 cites W2139004845 @default.
- W2015382308 cites W2141778672 @default.
- W2015382308 cites W2145471369 @default.
- W2015382308 cites W2146347034 @default.
- W2015382308 cites W2146922714 @default.
- W2015382308 cites W2149701148 @default.
- W2015382308 cites W2153352508 @default.
- W2015382308 cites W2153359643 @default.
- W2015382308 cites W2164240607 @default.
- W2015382308 cites W2165360511 @default.
- W2015382308 cites W2166550260 @default.
- W2015382308 cites W2171006004 @default.
- W2015382308 cites W2206073599 @default.
- W2015382308 cites W2305500926 @default.
- W2015382308 cites W4238270754 @default.
- W2015382308 cites W4240969190 @default.
- W2015382308 cites W4247418383 @default.
- W2015382308 doi "https://doi.org/10.1021/bi8019965" @default.
- W2015382308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19132923" @default.
- W2015382308 hasPublicationYear "2009" @default.
- W2015382308 type Work @default.
- W2015382308 sameAs 2015382308 @default.
- W2015382308 citedByCount "26" @default.
- W2015382308 countsByYear W20153823082012 @default.
- W2015382308 countsByYear W20153823082013 @default.
- W2015382308 countsByYear W20153823082014 @default.
- W2015382308 countsByYear W20153823082015 @default.
- W2015382308 countsByYear W20153823082017 @default.