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- W2015408772 abstract "Growing mouse oocytes incompetent to mature were freed of attached granulosa cells at different stages of growth, and cultured in vitro in the presence of fibroblast monolayers and/or their products. In these culture conditions, although growth was arrested, isolated oocytes survived in vitro for several days, and finally resumed meiosis spontaneously, progressing up to metaphase I. The culture time length needed for in vitro acquisition of the capacity to mature was inversely related to the initial oocyte size at the time of isolation from granulosa cells, and closely corresponded to developmental timing of acquisition of such ability in vivo. We conclude that the acquisition of mouse oocyte competence to mature follows a definite time program, which is independent of the presence of granulosa cells and of heterologous cell contacts, at least within the developmental stages studied." @default.
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- W2015408772 date "1984-04-01" @default.
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- W2015408772 title "Early programming of maturation competence in mouse oogenesis" @default.
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- W2015408772 doi "https://doi.org/10.1016/0012-1606(84)90220-3" @default.
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