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• W2015423235 abstract "Charge density measurements and polarization modulation infrared reflection absorption spectroscopy were employed to investigate the spreading of small unilamellar vesicles of a dimyristoylphosphatidylcholine (DMPC)/cholesterol (7:3 molar ratio) mixture onto an Au (111) electrode surface. The electrochemical experiments demonstrated that vesicles fuse and spread onto the Au (111) electrode surface, forming a bilayer, at rational potentials −0.4 V < (E−Epzc) < 0.4 V or field strength <6×107 V m−1. Polarization modulation infrared reflection absorption spectroscopy experiments provided information concerning the conformation and orientation of the acyl chains of DMPC molecules. Deuterated DMPC was used to subtract the contribution of C-H stretching bands of cholesterol and of the polar head region of DMPC from spectra in the C-H stretching region. The absorption spectra of the C-H stretch bands in the acyl chains were determined in this way. The properties of the DMPC/cholesterol bilayer have been compared with the properties of a pure DMPC bilayer. The presence of 30% cholesterol gives a thicker and more fluid bilayer characterized by a lower capacity and lower tilt angle of the acyl chains." @default.
• W2015423235 created "2016-06-24" @default.
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• W2015423235 creator A5019836139 @default.
• W2015423235 creator A5063871487 @default.
• W2015423235 date "2005-07-01" @default.
• W2015423235 modified "2023-10-10" @default.
• W2015423235 title "Electrochemical and PM-IRRAS Studies of the Effect of Cholesterol on the Structure of a DMPC Bilayer Supported at an Au (111) Electrode Surface, Part 1: Properties of the Acyl Chains" @default.
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• W2015423235 doi "https://doi.org/10.1529/biophysj.104.058347" @default.
• W2015423235 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1366559" @default.
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