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• W2015437300 abstract "Abstract While chronic hypoglycaemia has been reported to increase unidirectional glucose transport across the blood–brain barrier (BBB) and to increase GLUT1 expression at the endothelium, the effect on steady‐state brain d ‐glucose and brain glycogen content is currently unknown. Brain glucose and glycogen concentrations were directly measured in vivo using localized 13 C magnetic resonance spectroscopy (MRS) following 12–14 days of hypoglycaemia. Brain glucose content was significantly increased by 48%, which is consistent with an increase in the maximal glucose transport rate, T max , by 58% compared with the sham‐treated animals. The localized 13 C NMR measurements of brain glucose were directly validated by comparison with biochemically determined brain glucose content after rapid focused microwave fixation (1.4 s at 4 kW). Both in vivo MRS and biochemical measurements implied that brain glycogen content was not affected by chronic hypoglycaemia, consistent with brain glucose being a major factor controlling brain glycogen content. We conclude that the increased glucose transporter expression in chronic hypoglycaemia leads to increased brain glucose content at a given level of glycaemia. Such increased brain glucose concentrations can result in a lowered glycaemic threshold of counter‐regulation observed in chronic hypoglycaemia." @default.
• W2015437300 created "2016-06-24" @default.
• W2015437300 creator A5029409294 @default.
• W2015437300 creator A5060942510 @default.
• W2015437300 date "2006-07-26" @default.
• W2015437300 modified "2023-10-13" @default.
• W2015437300 title "Effect of chronic hypoglycaemia on glucose concentration and glycogen content in rat brain: a localized¹³C NMR study" @default.
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• W2015437300 doi "https://doi.org/10.1111/j.1471-4159.2006.04115.x" @default.
• W2015437300 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1618787" @default.
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