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- W2015442731 abstract "A general strategy for the site-specific dual-labeling of proteins for single-molecule fluorescence resonance energy transfer is presented. A genetically encoded unnatural ketone amino acid was labeled with a hydroxylamine-containing fluorophore with high yield (>95%) and specificity. This methodology was used to construct dual-labeled T4 lysozyme variants, allowing the study of T4 lysozyme folding at single-molecule resolution. The presented strategy is anticipated to expand the scope of single-molecule protein structure and function studies." @default.
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- W2015442731 date "2008-12-09" @default.
- W2015442731 modified "2023-09-25" @default.
- W2015442731 title "A General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer" @default.
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- W2015442731 doi "https://doi.org/10.1021/ja807430h" @default.
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