Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015472510> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2015472510 endingPage "747" @default.
- W2015472510 startingPage "739" @default.
- W2015472510 abstract "The purpose of this study was to examine whether nitric oxide (NO) synthase dysfunction accompanied with decrease in tetrahydrobiopterin (BH4) content increases H2O2-induced endothelial cell death. Endothelial cell death was measured by the release of intracellular lactate dehydrogenase (LDH). Intracellular BH4 content was changed by pretreatment with 2,4-diamino-6-hydroxypyrimidine (DAHP), an inhibitor of GTP cyclohydrolase I, or pretreatment with sepiapterin, a substrate for the salvage pathway of BH4 synthesis, and the intracellular content was measured by high performance liquid chromatography equipped with a fluorescence detector. Moreover, production of superoxide was detected by a chemiluminescence technique using MCLA, a Cypridina luciferin analogue, for the superoxide-sensitive probe. Pretreatment with DAHP (10 mM) for 24 h decreased intracellular BH4 content to 14% and increased H2O2-induced cell death. The toxic effect of DAHP was reduced by co-pretreatment with sepiapterin (100 microM) or treatment with N(G)-nitro-L-arginine methyl ester (L-NAME, 1 mM), an inhibitor of NO synthase, but not by N(G)-methyl-L-arginine (L-NMA, 1 mM), the other inhibitor of NO synthase. Moreover, production of superoxide in endothelial cells induced by Ca2+-ionophore ionomycin (1 microM) increased by the pretreatment with DAHP, and the increase in superoxide production was blocked by L-NAME (1 mM) but not L-NMA (1 mM). Co-pretreatment with sepiapterin decreased the production of superoxide. These findings suggested that dysfunction of NO synthase with a decrease in BH4 content in endothelial cells produced superoxide instead of NO and increased the oxidative stress-induced endothelial cell death." @default.
- W2015472510 created "2016-06-24" @default.
- W2015472510 creator A5020898431 @default.
- W2015472510 creator A5022886115 @default.
- W2015472510 creator A5034568368 @default.
- W2015472510 creator A5046764083 @default.
- W2015472510 creator A5065131469 @default.
- W2015472510 date "1997-07-01" @default.
- W2015472510 modified "2023-09-23" @default.
- W2015472510 title "Acceleration of oxidative stress-induced endothelial cell death by nitric oxide synthase dysfunction accompanied with decrease in tetrahydrobiopterin content" @default.
- W2015472510 cites W1946153048 @default.
- W2015472510 cites W1967315269 @default.
- W2015472510 cites W1972592700 @default.
- W2015472510 cites W1975884700 @default.
- W2015472510 cites W1982021457 @default.
- W2015472510 cites W1982581996 @default.
- W2015472510 cites W1982584549 @default.
- W2015472510 cites W1994389040 @default.
- W2015472510 cites W1996642263 @default.
- W2015472510 cites W1998507456 @default.
- W2015472510 cites W2000507650 @default.
- W2015472510 cites W2028676745 @default.
- W2015472510 cites W2037854429 @default.
- W2015472510 cites W2040398874 @default.
- W2015472510 cites W2055205809 @default.
- W2015472510 cites W2061382541 @default.
- W2015472510 cites W2076757239 @default.
- W2015472510 cites W2078671924 @default.
- W2015472510 cites W2081928955 @default.
- W2015472510 cites W2085587120 @default.
- W2015472510 cites W2157532432 @default.
- W2015472510 cites W2176832201 @default.
- W2015472510 cites W2213248838 @default.
- W2015472510 cites W2402886149 @default.
- W2015472510 doi "https://doi.org/10.1016/s0024-3205(97)00538-9" @default.
- W2015472510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9252248" @default.
- W2015472510 hasPublicationYear "1997" @default.
- W2015472510 type Work @default.
- W2015472510 sameAs 2015472510 @default.
- W2015472510 citedByCount "33" @default.
- W2015472510 countsByYear W20154725102019 @default.
- W2015472510 crossrefType "journal-article" @default.
- W2015472510 hasAuthorship W2015472510A5020898431 @default.
- W2015472510 hasAuthorship W2015472510A5022886115 @default.
- W2015472510 hasAuthorship W2015472510A5034568368 @default.
- W2015472510 hasAuthorship W2015472510A5046764083 @default.
- W2015472510 hasAuthorship W2015472510A5065131469 @default.
- W2015472510 hasConcept C123012128 @default.
- W2015472510 hasConcept C178790620 @default.
- W2015472510 hasConcept C181199279 @default.
- W2015472510 hasConcept C185592680 @default.
- W2015472510 hasConcept C202751555 @default.
- W2015472510 hasConcept C2775838275 @default.
- W2015472510 hasConcept C2776145790 @default.
- W2015472510 hasConcept C2776151105 @default.
- W2015472510 hasConcept C2776989580 @default.
- W2015472510 hasConcept C2777622882 @default.
- W2015472510 hasConcept C2780795997 @default.
- W2015472510 hasConcept C519581460 @default.
- W2015472510 hasConcept C55493867 @default.
- W2015472510 hasConcept C79879829 @default.
- W2015472510 hasConceptScore W2015472510C123012128 @default.
- W2015472510 hasConceptScore W2015472510C178790620 @default.
- W2015472510 hasConceptScore W2015472510C181199279 @default.
- W2015472510 hasConceptScore W2015472510C185592680 @default.
- W2015472510 hasConceptScore W2015472510C202751555 @default.
- W2015472510 hasConceptScore W2015472510C2775838275 @default.
- W2015472510 hasConceptScore W2015472510C2776145790 @default.
- W2015472510 hasConceptScore W2015472510C2776151105 @default.
- W2015472510 hasConceptScore W2015472510C2776989580 @default.
- W2015472510 hasConceptScore W2015472510C2777622882 @default.
- W2015472510 hasConceptScore W2015472510C2780795997 @default.
- W2015472510 hasConceptScore W2015472510C519581460 @default.
- W2015472510 hasConceptScore W2015472510C55493867 @default.
- W2015472510 hasConceptScore W2015472510C79879829 @default.
- W2015472510 hasIssue "7" @default.
- W2015472510 hasLocation W20154725101 @default.
- W2015472510 hasLocation W20154725102 @default.
- W2015472510 hasOpenAccess W2015472510 @default.
- W2015472510 hasPrimaryLocation W20154725101 @default.
- W2015472510 hasRelatedWork W1985611674 @default.
- W2015472510 hasRelatedWork W2018643804 @default.
- W2015472510 hasRelatedWork W2024138274 @default.
- W2015472510 hasRelatedWork W2056311899 @default.
- W2015472510 hasRelatedWork W2125442230 @default.
- W2015472510 hasRelatedWork W2388905353 @default.
- W2015472510 hasRelatedWork W2417154303 @default.
- W2015472510 hasRelatedWork W435811499 @default.
- W2015472510 hasRelatedWork W46191381 @default.
- W2015472510 hasRelatedWork W72147876 @default.
- W2015472510 hasVolume "61" @default.
- W2015472510 isParatext "false" @default.
- W2015472510 isRetracted "false" @default.
- W2015472510 magId "2015472510" @default.
- W2015472510 workType "article" @default.