FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015486355> ?p ?o ?g. }

• W2015486355 endingPage "23" @default.
• W2015486355 startingPage "11" @default.
• W2015486355 abstract "During the last decade, recombinant AAVs have become of increasing interest for gene therapy. Clinical trials have been conducted following promising in vivo evaluations, thus leading laboratories to adapt their production systems for larger and higher quality demands. Classical transfection protocols seem difficult and cumbersome to adapt to a bioreactor scale. The use of stable producer cells appears as an attractive alternative, as this system requires only a single infection step to induce rAAV production. Furthermore, the switch to a serum-free medium is an interesting strategy to increase the biosafety level to satisfy clinical grade requirements for gene therapy products. Here, we have combined both approaches and evaluated different rAAV producer clones in a serum-free medium. We first evaluated the cell growth in a serum-free medium and then did a partial optimisation of the medium composition to obtain vector yields as close as possible to the yields obtained in a classical serum containing medium. Different helper viruses, multiplicity of infection, times of infection and harvest have been compared in small scale cultures in order to determine the optimal settings which were then transferred and evaluated in suspension cultures in spinner flasks. The yields obtained in this system were similar to or at most 2 times lower than those obtained in a serum-containing medium. The scale-up of such a production system as well as the use of high cell density perfusion culture systems will probably lead to considerably higher yields than those obtained in a classical process." @default.
• W2015486355 created "2016-06-24" @default.
• W2015486355 creator A5008391387 @default.
• W2015486355 creator A5027019110 @default.
• W2015486355 creator A5041829016 @default.
• W2015486355 creator A5043124146 @default.
• W2015486355 date "2005-09-01" @default.
• W2015486355 modified "2023-10-08" @default.
• W2015486355 title "Evaluation of a Serum-free Medium for the Production of rAAV-2 using HeLa Derived Producer Cells" @default.
• W2015486355 cites W121665557 @default.
• W2015486355 cites W140890592 @default.
• W2015486355 cites W1488961672 @default.
• W2015486355 cites W1508946059 @default.
• W2015486355 cites W1619761819 @default.
• W2015486355 cites W1868673470 @default.
• W2015486355 cites W1975528602 @default.
• W2015486355 cites W1997403299 @default.
• W2015486355 cites W1998853863 @default.
• W2015486355 cites W2001657410 @default.
• W2015486355 cites W2005344589 @default.
• W2015486355 cites W2009310029 @default.
• W2015486355 cites W2009822728 @default.
• W2015486355 cites W2019814860 @default.
• W2015486355 cites W2026505509 @default.
• W2015486355 cites W2026854040 @default.
• W2015486355 cites W2030384860 @default.
• W2015486355 cites W2033842492 @default.
• W2015486355 cites W2041892905 @default.
• W2015486355 cites W2042131554 @default.
• W2015486355 cites W2058234735 @default.
• W2015486355 cites W2062622002 @default.
• W2015486355 cites W2068065419 @default.
• W2015486355 cites W2068506694 @default.
• W2015486355 cites W2074487444 @default.
• W2015486355 cites W2075808672 @default.
• W2015486355 cites W2076595139 @default.
• W2015486355 cites W2080792225 @default.
• W2015486355 cites W2081079533 @default.
• W2015486355 cites W2091924039 @default.
• W2015486355 cites W2101605345 @default.
• W2015486355 cites W2105279765 @default.
• W2015486355 cites W2107856434 @default.
• W2015486355 cites W2112931437 @default.
• W2015486355 cites W2130314959 @default.
• W2015486355 cites W2148223197 @default.
• W2015486355 doi "https://doi.org/10.1007/s10616-005-5361-z" @default.
• W2015486355 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3449747" @default.
• W2015486355 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19003059" @default.
• W2015486355 hasPublicationYear "2005" @default.
• W2015486355 type Work @default.
• W2015486355 sameAs 2015486355 @default.
• W2015486355 citedByCount "16" @default.
• W2015486355 countsByYear W20154863552013 @default.
• W2015486355 countsByYear W20154863552015 @default.
• W2015486355 countsByYear W20154863552017 @default.
• W2015486355 countsByYear W20154863552018 @default.
• W2015486355 countsByYear W20154863552019 @default.
• W2015486355 countsByYear W20154863552023 @default.
• W2015486355 crossrefType "journal-article" @default.
• W2015486355 hasAuthorship W2015486355A5008391387 @default.
• W2015486355 hasAuthorship W2015486355A5027019110 @default.
• W2015486355 hasAuthorship W2015486355A5041829016 @default.
• W2015486355 hasAuthorship W2015486355A5043124146 @default.
• W2015486355 hasBestOaLocation W20154863552 @default.
• W2015486355 hasConcept C104317684 @default.
• W2015486355 hasConcept C111599444 @default.
• W2015486355 hasConcept C127413603 @default.
• W2015486355 hasConcept C136229726 @default.
• W2015486355 hasConcept C139149728 @default.
• W2015486355 hasConcept C147789679 @default.
• W2015486355 hasConcept C150903083 @default.
• W2015486355 hasConcept C168170006 @default.
• W2015486355 hasConcept C183696295 @default.
• W2015486355 hasConcept C185592680 @default.
• W2015486355 hasConcept C203014093 @default.
• W2015486355 hasConcept C203233990 @default.
• W2015486355 hasConcept C207001950 @default.
• W2015486355 hasConcept C2777366897 @default.
• W2015486355 hasConcept C52384281 @default.
• W2015486355 hasConcept C54009773 @default.
• W2015486355 hasConcept C54355233 @default.
• W2015486355 hasConcept C55493867 @default.
• W2015486355 hasConcept C59822182 @default.
• W2015486355 hasConcept C70721500 @default.
• W2015486355 hasConcept C71924100 @default.
• W2015486355 hasConcept C81885089 @default.
• W2015486355 hasConcept C86803240 @default.
• W2015486355 hasConceptScore W2015486355C104317684 @default.
• W2015486355 hasConceptScore W2015486355C111599444 @default.
• W2015486355 hasConceptScore W2015486355C127413603 @default.
• W2015486355 hasConceptScore W2015486355C136229726 @default.
• W2015486355 hasConceptScore W2015486355C139149728 @default.
• W2015486355 hasConceptScore W2015486355C147789679 @default.
• W2015486355 hasConceptScore W2015486355C150903083 @default.
• W2015486355 hasConceptScore W2015486355C168170006 @default.
• W2015486355 hasConceptScore W2015486355C183696295 @default.
• W2015486355 hasConceptScore W2015486355C185592680 @default.
• W2015486355 hasConceptScore W2015486355C203014093 @default.

• next