FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015486512> ?p ?o ?g. }

• W2015486512 endingPage "160" @default.
• W2015486512 startingPage "153" @default.
• W2015486512 abstract "Adenovirus (Ad) is one of the viral vectors most widely used for gene delivery. The virus, however, has serious shortcomings such as immunogenicity, promiscuous tropism, and the inability to efficiently infect certain types of cells. The goal of this study was to improve the ability of an Ad-based vector to efficiently transform cells that lack the native coxsackie-adenovirus receptor (CAR(-)) by modifying the virus with CPP-PEG conjugates.The vector was produced by PEGylating Ad, which packages a lacZ reporter gene, and then conjugating CPPs to form CPP-PEG-Ad particles. The study compared the effectiveness of four different CPPs: Pen, Tat, Pep1, and pArg. The effects of CPP amount per virus, degree of PEGylation, and PEG molecular weight on transduction efficiency were studied on CAR(-) NIH/3T3 cells.CPP-PEG-Ad particles transduced CAR(-) cells significantly better than unmodified Ad. Pen, the most effective CPP, produced an 80-fold improvement in transduction compared to the unmodified virus. The Pen peptide utilized a combination of electrostatic and hydrophobic interactions with the cell membrane to maximize cellular association while the other CPPs used only electrostatic or hydrophobic interactions but not both. Lastly, higher degrees of PEGylation, which prompted PEG to adopt a brush conformation, resulted in more efficient CPP-PEG-Ad particles because of both better conjugation of CPPs to the PEGylated virus and better exposure of the conjugated CPPs on the surface of the particle.CPP-PEG-Ad particles efficiently deliver genes to cells that Ad alone would not efficiently infect, thereby extending potential gene therapy treatments to a much broader range of cell types and diseases." @default.
• W2015486512 created "2016-06-24" @default.
• W2015486512 creator A5002135195 @default.
• W2015486512 creator A5021409765 @default.
• W2015486512 creator A5058966466 @default.
• W2015486512 creator A5069759608 @default.
• W2015486512 date "2015-04-01" @default.
• W2015486512 modified "2023-09-25" @default.
• W2015486512 title "Effects of cell-penetrating peptides on transduction efficiency of PEGylated adenovirus" @default.
• W2015486512 cites W1547929361 @default.
• W2015486512 cites W1908149672 @default.
• W2015486512 cites W1970767319 @default.
• W2015486512 cites W1982805293 @default.
• W2015486512 cites W1984376606 @default.
• W2015486512 cites W1992095148 @default.
• W2015486512 cites W1995276829 @default.
• W2015486512 cites W2001493784 @default.
• W2015486512 cites W2006370393 @default.
• W2015486512 cites W2008033906 @default.
• W2015486512 cites W2017614842 @default.
• W2015486512 cites W2019921823 @default.
• W2015486512 cites W2022429082 @default.
• W2015486512 cites W2024330925 @default.
• W2015486512 cites W2025616153 @default.
• W2015486512 cites W2030002132 @default.
• W2015486512 cites W2036939362 @default.
• W2015486512 cites W2037305018 @default.
• W2015486512 cites W2050748316 @default.
• W2015486512 cites W2051758487 @default.
• W2015486512 cites W2054790400 @default.
• W2015486512 cites W2064761355 @default.
• W2015486512 cites W2075827326 @default.
• W2015486512 cites W2077102218 @default.
• W2015486512 cites W2078681344 @default.
• W2015486512 cites W2079603374 @default.
• W2015486512 cites W2086752617 @default.
• W2015486512 cites W2087503461 @default.
• W2015486512 cites W2092327932 @default.
• W2015486512 cites W2094492208 @default.
• W2015486512 cites W2107910518 @default.
• W2015486512 cites W2111496511 @default.
• W2015486512 cites W2120654936 @default.
• W2015486512 cites W2125450037 @default.
• W2015486512 cites W2143598717 @default.
• W2015486512 cites W2146144253 @default.
• W2015486512 cites W2146261786 @default.
• W2015486512 cites W2316811173 @default.
• W2015486512 cites W339058748 @default.
• W2015486512 cites W4210354241 @default.
• W2015486512 cites W4239028853 @default.
• W2015486512 doi "https://doi.org/10.1016/j.biopha.2015.02.015" @default.
• W2015486512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25960231" @default.
• W2015486512 hasPublicationYear "2015" @default.
• W2015486512 type Work @default.
• W2015486512 sameAs 2015486512 @default.
• W2015486512 citedByCount "20" @default.
• W2015486512 countsByYear W20154865122015 @default.
• W2015486512 countsByYear W20154865122016 @default.
• W2015486512 countsByYear W20154865122017 @default.
• W2015486512 countsByYear W20154865122018 @default.
• W2015486512 countsByYear W20154865122019 @default.
• W2015486512 countsByYear W20154865122020 @default.
• W2015486512 countsByYear W20154865122022 @default.
• W2015486512 countsByYear W20154865122023 @default.
• W2015486512 crossrefType "journal-article" @default.
• W2015486512 hasAuthorship W2015486512A5002135195 @default.
• W2015486512 hasAuthorship W2015486512A5021409765 @default.
• W2015486512 hasAuthorship W2015486512A5058966466 @default.
• W2015486512 hasAuthorship W2015486512A5069759608 @default.
• W2015486512 hasConcept C10138342 @default.
• W2015486512 hasConcept C104317684 @default.
• W2015486512 hasConcept C12554922 @default.
• W2015486512 hasConcept C135983454 @default.
• W2015486512 hasConcept C1491633281 @default.
• W2015486512 hasConcept C150194340 @default.
• W2015486512 hasConcept C15152581 @default.
• W2015486512 hasConcept C159047783 @default.
• W2015486512 hasConcept C161733203 @default.
• W2015486512 hasConcept C162324750 @default.
• W2015486512 hasConcept C185592680 @default.
• W2015486512 hasConcept C203014093 @default.
• W2015486512 hasConcept C2522874641 @default.
• W2015486512 hasConcept C2776929087 @default.
• W2015486512 hasConcept C2776964284 @default.
• W2015486512 hasConcept C2779149719 @default.
• W2015486512 hasConcept C2779281246 @default.
• W2015486512 hasConcept C2780868878 @default.
• W2015486512 hasConcept C32470452 @default.
• W2015486512 hasConcept C40767141 @default.
• W2015486512 hasConcept C54009773 @default.
• W2015486512 hasConcept C54400483 @default.
• W2015486512 hasConcept C55493867 @default.
• W2015486512 hasConcept C86803240 @default.
• W2015486512 hasConcept C8891405 @default.
• W2015486512 hasConcept C95444343 @default.
• W2015486512 hasConceptScore W2015486512C10138342 @default.
• W2015486512 hasConceptScore W2015486512C104317684 @default.
• W2015486512 hasConceptScore W2015486512C12554922 @default.

• next