Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015489056> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2015489056 endingPage "626" @default.
- W2015489056 startingPage "615" @default.
- W2015489056 abstract "Various factors most likely to influence the plasma protein binding of azosemide to 4% human serum albumin (HSA) were evaluated using equilibrium dialysis at the initial azosemide concentration of 10 μg mL−1. It took approximately 8h of incubation to reach an equilibrium between 4% HSA and isotonic phosphate buffer of pH 7.4 containing 3% dextran (the ‘buffer’) using a Spectra/Por 2 membrane (molecular weight cut-off 12000–14000) in a water bath shaker kept at 37°C and a rate of 50 oscillations min−1. Azosemide was fairly stable both in 4% HSA and in the ‘buffer’ for up to 24h. The binding of azosemide to 4% HSA was constant (95.5 ± 0.142%) at azosemide concentrations ranging from 5 to 100 μg mL−1. However, the extent of binding was dependent on HSA concentration: the values were 88.4, 91.0, 92.2, 94.2, 94.9, 94.9, and 94.9% at albumin concentrations of 0.5, 1, 2, 3, 4, 5, and 6% respectively. The binding was also dependent on incubation temperature; the binding values were 97.0, 94.9, and 94.9% when incubated at 6, 28, and 37°C, respectively. The binding of azosemide was also influenced by buffers containing various chloride ion concentrations and buffer pHs. The binding values were 95.3, 94.9, and 93.6% for the chloride ion concentrations of 0, 0.249, and 0.546%, respectively, and the unbound values were 6.8, 5.1, 3.8, 3.4, and 3.3% for buffer pHs of 5.8, 6.4, 7.0, 7.4, and 8.0, respectively. The binding of azosemide was independent of the quantity of heparin (up to 40 UmL−1), AAG (up to 0.16%), sodium azide (NaN3, up to 5%), its metabolite, Ml (up to 10 μg mL−1), and anticoagulants (EDTA and citrate)." @default.
- W2015489056 created "2016-06-24" @default.
- W2015489056 creator A5017370039 @default.
- W2015489056 creator A5056801261 @default.
- W2015489056 date "1995-10-01" @default.
- W2015489056 modified "2023-09-27" @default.
- W2015489056 title "Factors influencing the protein binding of azosemide using an equilibrium dialysis technique" @default.
- W2015489056 cites W1965789860 @default.
- W2015489056 cites W1972387119 @default.
- W2015489056 cites W1976655639 @default.
- W2015489056 cites W1980903735 @default.
- W2015489056 cites W1985801662 @default.
- W2015489056 cites W1991613456 @default.
- W2015489056 cites W1993721232 @default.
- W2015489056 cites W1993926418 @default.
- W2015489056 cites W2006659089 @default.
- W2015489056 cites W2010502743 @default.
- W2015489056 cites W2010568702 @default.
- W2015489056 cites W2012197537 @default.
- W2015489056 cites W2016665392 @default.
- W2015489056 cites W2021471240 @default.
- W2015489056 cites W2022559430 @default.
- W2015489056 cites W2026567219 @default.
- W2015489056 cites W2032877182 @default.
- W2015489056 cites W2036372142 @default.
- W2015489056 cites W2045532705 @default.
- W2015489056 cites W2048352275 @default.
- W2015489056 cites W2058480691 @default.
- W2015489056 cites W2062250291 @default.
- W2015489056 cites W2062632363 @default.
- W2015489056 cites W2068213979 @default.
- W2015489056 cites W2071550682 @default.
- W2015489056 cites W2073122425 @default.
- W2015489056 cites W2077808904 @default.
- W2015489056 cites W2088004174 @default.
- W2015489056 cites W2094381112 @default.
- W2015489056 cites W2135999585 @default.
- W2015489056 cites W2136914926 @default.
- W2015489056 cites W2149156314 @default.
- W2015489056 cites W2420252447 @default.
- W2015489056 cites W2431515370 @default.
- W2015489056 cites W2435688793 @default.
- W2015489056 cites W2885086560 @default.
- W2015489056 cites W34440838 @default.
- W2015489056 doi "https://doi.org/10.1002/bdd.2510160709" @default.
- W2015489056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8785384" @default.
- W2015489056 hasPublicationYear "1995" @default.
- W2015489056 type Work @default.
- W2015489056 sameAs 2015489056 @default.
- W2015489056 citedByCount "15" @default.
- W2015489056 countsByYear W20154890562013 @default.
- W2015489056 countsByYear W20154890562014 @default.
- W2015489056 countsByYear W20154890562021 @default.
- W2015489056 crossrefType "journal-article" @default.
- W2015489056 hasAuthorship W2015489056A5017370039 @default.
- W2015489056 hasAuthorship W2015489056A5056801261 @default.
- W2015489056 hasConcept C185592680 @default.
- W2015489056 hasConcept C25642318 @default.
- W2015489056 hasConcept C2778597219 @default.
- W2015489056 hasConcept C43617362 @default.
- W2015489056 hasConcept C51639874 @default.
- W2015489056 hasConcept C55493867 @default.
- W2015489056 hasConceptScore W2015489056C185592680 @default.
- W2015489056 hasConceptScore W2015489056C25642318 @default.
- W2015489056 hasConceptScore W2015489056C2778597219 @default.
- W2015489056 hasConceptScore W2015489056C43617362 @default.
- W2015489056 hasConceptScore W2015489056C51639874 @default.
- W2015489056 hasConceptScore W2015489056C55493867 @default.
- W2015489056 hasIssue "7" @default.
- W2015489056 hasLocation W20154890561 @default.
- W2015489056 hasLocation W20154890562 @default.
- W2015489056 hasOpenAccess W2015489056 @default.
- W2015489056 hasPrimaryLocation W20154890561 @default.
- W2015489056 hasRelatedWork W1979616565 @default.
- W2015489056 hasRelatedWork W1992682118 @default.
- W2015489056 hasRelatedWork W1993478978 @default.
- W2015489056 hasRelatedWork W2002970128 @default.
- W2015489056 hasRelatedWork W2069842851 @default.
- W2015489056 hasRelatedWork W2077355290 @default.
- W2015489056 hasRelatedWork W2091343852 @default.
- W2015489056 hasRelatedWork W2102415270 @default.
- W2015489056 hasRelatedWork W2125275954 @default.
- W2015489056 hasRelatedWork W2741865982 @default.
- W2015489056 hasVolume "16" @default.
- W2015489056 isParatext "false" @default.
- W2015489056 isRetracted "false" @default.
- W2015489056 magId "2015489056" @default.
- W2015489056 workType "article" @default.