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- W2015489145 abstract "This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In contrast, no changes were found when samples were incubated with the O-linked glycosylation-specific O-glycosidase. Since the GH-N molecule lacks consensus sequences for N-linked glycosylation, our findings suggest that GH genes other than hGH-N are expressed in the human pituitary gland." @default.
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- W2015489145 date "2000-01-01" @default.
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- W2015489145 title "N-Glycosylated Variants of Growth Hormone in Human Pituitary Extracts" @default.
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- W2015489145 doi "https://doi.org/10.1159/000023512" @default.
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