FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015510635> ?p ?o ?g. }

• W2015510635 endingPage "138" @default.
• W2015510635 startingPage "129" @default.
• W2015510635 abstract "A synthetic gene encoding the 39-amino-acid (aa) potato carboxypeptidase inhibitor IIa (PCI-IIa) has been constructed and expressed using the secretion vector, pIN-III-ompA-3, fused in frame to the OmpA signal peptide-encoding sequence. Recombinant Escherichia coli secreted a PCI with 10 additional aa at the N terminus (rePCI+10). These extra aa were removed by site-directed mutagenesis giving a PCI with no additional aa (rePCI), as shown by fast atom bombardment mass spectrometry (Mr 4295). The two forms of rePCI were found almost exclusively in the culture medium, not in the periplasmic space, as would be expected from OmpA signal peptide fusions. Both rePCI+10 and rePCI are biologically active and react strongly with serum raised against PCI from potato. A method for the purification of rePCI to homogeneity has been developed. The purified rePCI shows a Ki for carboxypeptidase A within the range of the natural PCI-IIa (1.5–2.7 nM). These results indicate that both rePCI+10 and rePCI are properly folded and that their three disulfide bridges are correctly formed. Together with previous reports, our results show that fusion to a secretion signal peptide is an effective way of producing small proteins containing disulfide bridges in a biologically active form." @default.
• W2015510635 created "2016-06-24" @default.
• W2015510635 creator A5000799305 @default.
• W2015510635 creator A5026144421 @default.
• W2015510635 creator A5072119318 @default.
• W2015510635 date "1992-07-01" @default.
• W2015510635 modified "2023-10-02" @default.
• W2015510635 title "Expression of a synthetic gene encoding potato carboxypeptidase inhibitor using a bacterial secretion vector" @default.
• W2015510635 cites W1124321114 @default.
• W2015510635 cites W1499276444 @default.
• W2015510635 cites W1516910236 @default.
• W2015510635 cites W1535133733 @default.
• W2015510635 cites W1623259890 @default.
• W2015510635 cites W1967507598 @default.
• W2015510635 cites W1968929239 @default.
• W2015510635 cites W1982031046 @default.
• W2015510635 cites W1984640298 @default.
• W2015510635 cites W2000905858 @default.
• W2015510635 cites W2006478561 @default.
• W2015510635 cites W2012126472 @default.
• W2015510635 cites W2013491898 @default.
• W2015510635 cites W2019414320 @default.
• W2015510635 cites W2021852264 @default.
• W2015510635 cites W2027497003 @default.
• W2015510635 cites W2028622989 @default.
• W2015510635 cites W2044772822 @default.
• W2015510635 cites W2050499271 @default.
• W2015510635 cites W2054698853 @default.
• W2015510635 cites W2057190076 @default.
• W2015510635 cites W2063982283 @default.
• W2015510635 cites W2070865589 @default.
• W2015510635 cites W2082228480 @default.
• W2015510635 cites W2086733743 @default.
• W2015510635 cites W2092048434 @default.
• W2015510635 cites W2110256765 @default.
• W2015510635 cites W2138721124 @default.
• W2015510635 cites W2140422547 @default.
• W2015510635 cites W2414435754 @default.
• W2015510635 cites W4293247451 @default.
• W2015510635 cites W4296765338 @default.
• W2015510635 doi "https://doi.org/10.1016/0378-1119(92)90508-m" @default.
• W2015510635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1634110" @default.
• W2015510635 hasPublicationYear "1992" @default.
• W2015510635 type Work @default.
• W2015510635 sameAs 2015510635 @default.
• W2015510635 citedByCount "39" @default.
• W2015510635 countsByYear W20155106352022 @default.
• W2015510635 crossrefType "journal-article" @default.
• W2015510635 hasAuthorship W2015510635A5000799305 @default.
• W2015510635 hasAuthorship W2015510635A5026144421 @default.
• W2015510635 hasAuthorship W2015510635A5072119318 @default.
• W2015510635 hasConcept C104317684 @default.
• W2015510635 hasConcept C108477463 @default.
• W2015510635 hasConcept C10858879 @default.
• W2015510635 hasConcept C123894998 @default.
• W2015510635 hasConcept C153911025 @default.
• W2015510635 hasConcept C167625842 @default.
• W2015510635 hasConcept C181199279 @default.
• W2015510635 hasConcept C201663137 @default.
• W2015510635 hasConcept C2779281246 @default.
• W2015510635 hasConcept C33161422 @default.
• W2015510635 hasConcept C40767141 @default.
• W2015510635 hasConcept C49039625 @default.
• W2015510635 hasConcept C515207424 @default.
• W2015510635 hasConcept C547475151 @default.
• W2015510635 hasConcept C55493867 @default.
• W2015510635 hasConcept C86803240 @default.
• W2015510635 hasConceptScore W2015510635C104317684 @default.
• W2015510635 hasConceptScore W2015510635C108477463 @default.
• W2015510635 hasConceptScore W2015510635C10858879 @default.
• W2015510635 hasConceptScore W2015510635C123894998 @default.
• W2015510635 hasConceptScore W2015510635C153911025 @default.
• W2015510635 hasConceptScore W2015510635C167625842 @default.
• W2015510635 hasConceptScore W2015510635C181199279 @default.
• W2015510635 hasConceptScore W2015510635C201663137 @default.
• W2015510635 hasConceptScore W2015510635C2779281246 @default.
• W2015510635 hasConceptScore W2015510635C33161422 @default.
• W2015510635 hasConceptScore W2015510635C40767141 @default.
• W2015510635 hasConceptScore W2015510635C49039625 @default.
• W2015510635 hasConceptScore W2015510635C515207424 @default.
• W2015510635 hasConceptScore W2015510635C547475151 @default.
• W2015510635 hasConceptScore W2015510635C55493867 @default.
• W2015510635 hasConceptScore W2015510635C86803240 @default.
• W2015510635 hasIssue "2" @default.
• W2015510635 hasLocation W20155106351 @default.
• W2015510635 hasLocation W20155106352 @default.
• W2015510635 hasOpenAccess W2015510635 @default.
• W2015510635 hasPrimaryLocation W20155106351 @default.
• W2015510635 hasRelatedWork W1917535562 @default.
• W2015510635 hasRelatedWork W2012604733 @default.
• W2015510635 hasRelatedWork W2021944202 @default.
• W2015510635 hasRelatedWork W2049643006 @default.
• W2015510635 hasRelatedWork W2087858067 @default.
• W2015510635 hasRelatedWork W2143779684 @default.
• W2015510635 hasRelatedWork W2147771743 @default.
• W2015510635 hasRelatedWork W4220675549 @default.
• W2015510635 hasRelatedWork W4248558637 @default.
• W2015510635 hasRelatedWork W4364352320 @default.

• next