Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015514654> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2015514654 endingPage "146" @default.
- W2015514654 startingPage "141" @default.
- W2015514654 abstract "Affinity chromatography is used for the purification of diagnostic polyclonal antibodies in order to ensure specificity. Most commonly, activated bead-formed agarose or its derivatives are used as gel matrices. Alternative matrix materials have been described, but as yet they do not appear to offer important advantages. In this study, pulverized polystyrene (PS 158K, BASF, Mannheim, Germany) was used as a solid phase for the immobilisation of bovine immunoglobulins (Ig). Affinity chromatography was performed using these coated polystyrene beads as the column matrix material in the purification of anti-bovine Ig. The polystyrene binding capacity for the different bovine Ig classes was compared using the Mancini single radial immunodiffusion technique, and ELISA procedures were used to monitor the antibody reactivity of purified and unpurified antibodies. The degree of purification was comparable to the most commonly used procedure using gel matrices from activated bead-formed agarose (e.g. CNBr-activated Sepharose 4B, Pharmacia/LKB Biotechnology, Uppsala, Sweden), but the antibody yield per ml column volume was distinctly lower. In order to raise the yield, such polystyrene bead columns with immobilized antigen can be re-used without loss of activity or larger column volumes can be used to raise the binding capacity. The polystyrene material is quite durable, chemically and immunologically inert and has a long shelf life. We conclude that polystyrene based affinity chromatography is efficient, simple and cheap." @default.
- W2015514654 created "2016-06-24" @default.
- W2015514654 creator A5004669450 @default.
- W2015514654 creator A5009894983 @default.
- W2015514654 creator A5020558723 @default.
- W2015514654 creator A5059212140 @default.
- W2015514654 date "1996-08-01" @default.
- W2015514654 modified "2023-09-25" @default.
- W2015514654 title "Polystyrene as an affinity chromatography matrix for the purification of antibodies" @default.
- W2015514654 cites W1913298599 @default.
- W2015514654 cites W2017193031 @default.
- W2015514654 cites W2032060707 @default.
- W2015514654 cites W2042379438 @default.
- W2015514654 cites W2052703223 @default.
- W2015514654 cites W2061035002 @default.
- W2015514654 cites W2074008752 @default.
- W2015514654 cites W2080779467 @default.
- W2015514654 cites W44305780 @default.
- W2015514654 doi "https://doi.org/10.1016/0022-1759(96)00142-1" @default.
- W2015514654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8765167" @default.
- W2015514654 hasPublicationYear "1996" @default.
- W2015514654 type Work @default.
- W2015514654 sameAs 2015514654 @default.
- W2015514654 citedByCount "14" @default.
- W2015514654 countsByYear W20155146542012 @default.
- W2015514654 countsByYear W20155146542014 @default.
- W2015514654 countsByYear W20155146542017 @default.
- W2015514654 countsByYear W20155146542018 @default.
- W2015514654 countsByYear W20155146542020 @default.
- W2015514654 countsByYear W20155146542021 @default.
- W2015514654 countsByYear W20155146542022 @default.
- W2015514654 crossrefType "journal-article" @default.
- W2015514654 hasAuthorship W2015514654A5004669450 @default.
- W2015514654 hasAuthorship W2015514654A5009894983 @default.
- W2015514654 hasAuthorship W2015514654A5020558723 @default.
- W2015514654 hasAuthorship W2015514654A5059212140 @default.
- W2015514654 hasConcept C106487976 @default.
- W2015514654 hasConcept C109386097 @default.
- W2015514654 hasConcept C159654299 @default.
- W2015514654 hasConcept C159985019 @default.
- W2015514654 hasConcept C178790620 @default.
- W2015514654 hasConcept C179247698 @default.
- W2015514654 hasConcept C181199279 @default.
- W2015514654 hasConcept C185592680 @default.
- W2015514654 hasConcept C192562407 @default.
- W2015514654 hasConcept C203014093 @default.
- W2015514654 hasConcept C2777922577 @default.
- W2015514654 hasConcept C2778476535 @default.
- W2015514654 hasConcept C2779881004 @default.
- W2015514654 hasConcept C43617362 @default.
- W2015514654 hasConcept C521977710 @default.
- W2015514654 hasConcept C55493867 @default.
- W2015514654 hasConcept C86803240 @default.
- W2015514654 hasConcept C956191 @default.
- W2015514654 hasConceptScore W2015514654C106487976 @default.
- W2015514654 hasConceptScore W2015514654C109386097 @default.
- W2015514654 hasConceptScore W2015514654C159654299 @default.
- W2015514654 hasConceptScore W2015514654C159985019 @default.
- W2015514654 hasConceptScore W2015514654C178790620 @default.
- W2015514654 hasConceptScore W2015514654C179247698 @default.
- W2015514654 hasConceptScore W2015514654C181199279 @default.
- W2015514654 hasConceptScore W2015514654C185592680 @default.
- W2015514654 hasConceptScore W2015514654C192562407 @default.
- W2015514654 hasConceptScore W2015514654C203014093 @default.
- W2015514654 hasConceptScore W2015514654C2777922577 @default.
- W2015514654 hasConceptScore W2015514654C2778476535 @default.
- W2015514654 hasConceptScore W2015514654C2779881004 @default.
- W2015514654 hasConceptScore W2015514654C43617362 @default.
- W2015514654 hasConceptScore W2015514654C521977710 @default.
- W2015514654 hasConceptScore W2015514654C55493867 @default.
- W2015514654 hasConceptScore W2015514654C86803240 @default.
- W2015514654 hasConceptScore W2015514654C956191 @default.
- W2015514654 hasIssue "2" @default.
- W2015514654 hasLocation W20155146541 @default.
- W2015514654 hasLocation W20155146542 @default.
- W2015514654 hasOpenAccess W2015514654 @default.
- W2015514654 hasPrimaryLocation W20155146541 @default.
- W2015514654 hasRelatedWork W1598059451 @default.
- W2015514654 hasRelatedWork W1640696459 @default.
- W2015514654 hasRelatedWork W2015514654 @default.
- W2015514654 hasRelatedWork W2017193031 @default.
- W2015514654 hasRelatedWork W2072967145 @default.
- W2015514654 hasRelatedWork W2379955423 @default.
- W2015514654 hasRelatedWork W2389862962 @default.
- W2015514654 hasRelatedWork W2397878069 @default.
- W2015514654 hasRelatedWork W2889109252 @default.
- W2015514654 hasRelatedWork W2040818558 @default.
- W2015514654 hasVolume "194" @default.
- W2015514654 isParatext "false" @default.
- W2015514654 isRetracted "false" @default.
- W2015514654 magId "2015514654" @default.
- W2015514654 workType "article" @default.