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• W2015555419 abstract "We present the solution conformation, determined by NMR spectroscopy, of a five-nucleotide RNA bulge loop. The bulge interrupts the stem of a 25-nucleotide RNA hairpin, and its sequence and flanking sequences are those of a conserved bulge from a Group I intron. The secondary structure of the bulge loop in the hairpin context is that predicted by the secondary structure prediction algorithm of Zuker. It differs, however, from the secondary structure deduced from sequence covariation of the bulge in the context of the functionally folded Group I introns and observed in the crystal structure of an independently folding domain of the Group I intron from Tetrahymena thermophila. This difference represents an exception to the heierarchical model of RNA folding in which preformed elements of secondary structure interact to form a tertiary structure. The three-dimensional structure of the bulge loop is characterized by discontinuous base stacking. Adjacent adenines stack with each other and with the flanking double helices. However, the position of the central uracil is not well defined by NOE distance constraints and is a point of discontinuity in the base stacking." @default.
• W2015555419 created "2016-06-24" @default.
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• W2015555419 date "1997-08-01" @default.
• W2015555419 modified "2023-09-26" @default.
• W2015555419 title "Solution Conformation of a Five-Nucleotide RNA Bulge Loop from a Group I Intron^," @default.
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• W2015555419 doi "https://doi.org/10.1021/bi9701540" @default.
• W2015555419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9254623" @default.
• W2015555419 hasPublicationYear "1997" @default.
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