FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015557944> ?p ?o ?g. }

• W2015557944 endingPage "161" @default.
• W2015557944 startingPage "150" @default.
• W2015557944 abstract "A microarray-based mix-and-measure, nonradioactive multiplex method with real-time detection was used for substrate identification, assay development, assay optimisation, and kinetic characterization of protein kinase A (PKA). The peptide arrays included either up to 140 serine/threonine-containing peptides or a concentration series of a smaller number of peptides. In comparison with existing singleplex assays, data quality was high, variation in assay conditions and reagent consumption were reduced considerably, and assay development could be accelerated because phosphorylation kinetics were monitored simultaneously on 4, 12, or 96 arrays. PKA was shown to phosphorylate many peptides containing known PKA phosphorylation sites as well as some new substrates. The kinetic behavior of the enzyme and the mechanism of inhibition by AMP–PNP, staurosporin, and PKA inhibitor peptide on the peptide microarray correlated well with data from homogeneous assays. Using this multiplex setup, we showed that the kinetic parameters of PKA and the potency of PKA inhibitors can be affected by the sequence of the peptide substrate. The technology enables kinetic monitoring of kinase activity in a multiplex setting such as a cell or tissue lysate. Finally, this high-throughput method allows fast identification of peptide substrates for serine/threonine kinases that are still uncharacterized." @default.
• W2015557944 created "2016-06-24" @default.
• W2015557944 creator A5060681084 @default.
• W2015557944 creator A5078376955 @default.
• W2015557944 creator A5088817453 @default.
• W2015557944 creator A5089466522 @default.
• W2015557944 date "2009-04-01" @default.
• W2015557944 modified "2023-09-29" @default.
• W2015557944 title "Peptide microarrays for detailed, high-throughput substrate identification, kinetic characterization, and inhibition studies on protein kinase A" @default.
• W2015557944 cites W1490238718 @default.
• W2015557944 cites W1501063278 @default.
• W2015557944 cites W1517257916 @default.
• W2015557944 cites W1528483543 @default.
• W2015557944 cites W1532142157 @default.
• W2015557944 cites W1557624269 @default.
• W2015557944 cites W1565643565 @default.
• W2015557944 cites W1614448865 @default.
• W2015557944 cites W1644548101 @default.
• W2015557944 cites W1945213815 @default.
• W2015557944 cites W1970075197 @default.
• W2015557944 cites W1974915358 @default.
• W2015557944 cites W1978696795 @default.
• W2015557944 cites W1980918328 @default.
• W2015557944 cites W1982855921 @default.
• W2015557944 cites W2007124698 @default.
• W2015557944 cites W2007694739 @default.
• W2015557944 cites W2015956088 @default.
• W2015557944 cites W2054271998 @default.
• W2015557944 cites W2055717246 @default.
• W2015557944 cites W2056184329 @default.
• W2015557944 cites W2064059972 @default.
• W2015557944 cites W2064685101 @default.
• W2015557944 cites W2067405360 @default.
• W2015557944 cites W2074631079 @default.
• W2015557944 cites W2076573118 @default.
• W2015557944 cites W2078374046 @default.
• W2015557944 cites W2081702130 @default.
• W2015557944 cites W2088547726 @default.
• W2015557944 cites W2091455173 @default.
• W2015557944 cites W2104244040 @default.
• W2015557944 cites W2134672568 @default.
• W2015557944 cites W2136126162 @default.
• W2015557944 cites W2138775700 @default.
• W2015557944 cites W2142337877 @default.
• W2015557944 cites W2143044209 @default.
• W2015557944 cites W2143630874 @default.
• W2015557944 cites W2159825665 @default.
• W2015557944 cites W2166884712 @default.
• W2015557944 cites W2168430186 @default.
• W2015557944 cites W2276934210 @default.
• W2015557944 cites W2291306536 @default.
• W2015557944 cites W2414435754 @default.
• W2015557944 cites W2916288174 @default.
• W2015557944 cites W2953375613 @default.
• W2015557944 cites W4211054902 @default.
• W2015557944 doi "https://doi.org/10.1016/j.ab.2009.01.022" @default.
• W2015557944 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19344656" @default.
• W2015557944 hasPublicationYear "2009" @default.
• W2015557944 type Work @default.
• W2015557944 sameAs 2015557944 @default.
• W2015557944 citedByCount "62" @default.
• W2015557944 countsByYear W20155579442012 @default.
• W2015557944 countsByYear W20155579442013 @default.
• W2015557944 countsByYear W20155579442014 @default.
• W2015557944 countsByYear W20155579442015 @default.
• W2015557944 countsByYear W20155579442016 @default.
• W2015557944 countsByYear W20155579442017 @default.
• W2015557944 countsByYear W20155579442018 @default.
• W2015557944 countsByYear W20155579442019 @default.
• W2015557944 countsByYear W20155579442020 @default.
• W2015557944 countsByYear W20155579442021 @default.
• W2015557944 countsByYear W20155579442022 @default.
• W2015557944 countsByYear W20155579442023 @default.
• W2015557944 crossrefType "journal-article" @default.
• W2015557944 hasAuthorship W2015557944A5060681084 @default.
• W2015557944 hasAuthorship W2015557944A5078376955 @default.
• W2015557944 hasAuthorship W2015557944A5088817453 @default.
• W2015557944 hasAuthorship W2015557944A5089466522 @default.
• W2015557944 hasConcept C104317684 @default.
• W2015557944 hasConcept C11960822 @default.
• W2015557944 hasConcept C150194340 @default.
• W2015557944 hasConcept C181199279 @default.
• W2015557944 hasConcept C184235292 @default.
• W2015557944 hasConcept C185592680 @default.
• W2015557944 hasConcept C18903297 @default.
• W2015557944 hasConcept C2775880066 @default.
• W2015557944 hasConcept C2776414213 @default.
• W2015557944 hasConcept C2777289219 @default.
• W2015557944 hasConcept C2779281246 @default.
• W2015557944 hasConcept C2781188995 @default.
• W2015557944 hasConcept C2910703447 @default.
• W2015557944 hasConcept C55493867 @default.
• W2015557944 hasConcept C60644358 @default.
• W2015557944 hasConcept C86803240 @default.
• W2015557944 hasConcept C95371953 @default.
• W2015557944 hasConcept C97029542 @default.
• W2015557944 hasConceptScore W2015557944C104317684 @default.
• W2015557944 hasConceptScore W2015557944C11960822 @default.

• next