FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015558270> ?p ?o ?g. }

• W2015558270 endingPage "293" @default.
• W2015558270 startingPage "279" @default.
• W2015558270 abstract "In the presence of 1–5 mM n-butyrate, murine leukemic L1210 cells cease proliferation and become arrested in the G1A compartment of the G1 phase. Cells in this compartment, in comparison with the remaining cells of the G1 phase (G1B), are characterized by low RNA content and more condensed chromatin. During unperturbed growth the cell residence times in G1A are of indeterminate duration (exponentially distributed); the half-time of L1210 cell residence in G1A is about 1.4 h. The effect of n-butyrate in arresting cells in G1A was concentration-dependent. However, the sensitivity of L1210 cells to this drug was markedly enhanced when cells were treated for longer than one generation (12 h). Cells arrested in G1A remained viable and when n-butyrate was removed, after a lag period, they resumed progression through the cycle. The effect of n-butyrate on cell progression through various parts of the cycle was studied in a stathmokinetic experiment. The rate of cell entrance into mitosis was decreased by 30, 60 and 110%, in the presence of 1, 2.5 and 5 mM n-butyrate respectively, thus indicating a slowdown in cell progression through G2 and S. The duration of G2 was prolonged by 20, 70 and 140% at 1, 2.5 and 5 mM n-butyrate respectively. The half-time of cell residence in G1A was increased by as much as 1.5-, 6.3- and 15.6-fold by 1, 2.5 and 5 mM n-butyrate. Progression through late G1 (G1B) was not affected at 1 mM, and could not be estimated at higher drug concentrations. The effects on cell cycle progression were evident 1 h after addition of n-butyrate. DNA in situ in nuclei of n-butyrate-treated cells had lowered (by 2–8 °C) stability to thermal denaturation and increased (by 15%) accessibility to DNase I. The decrease in DNA stability to heat was more pronounced when permealized cells were heated in the presence of 1 mM MgCl2 rather than EDTA. DNA in situ in the nuclei of n-butyrate-treated cells also showed decreased sensitivity to acid-induced denaturation. Changes in chromatin were seen in all cells, regardless of cell cycle phase, within the first hours after addition of n-butyrate. Mitotic cells, however, reacted to n-butyrate more rapidly than interphase cells. The observed changes in L1210 cells are most likely a consequence of histone modifications (acetylation of inner histones, dephosphorylation of histone H1) induced by n-butyrate." @default.
• W2015558270 created "2016-06-24" @default.
• W2015558270 creator A5013493640 @default.
• W2015558270 creator A5033158298 @default.
• W2015558270 creator A5051515853 @default.
• W2015558270 creator A5084175495 @default.
• W2015558270 date "1981-12-01" @default.
• W2015558270 modified "2023-10-16" @default.
• W2015558270 title "Effect of n-butyrate on cell cycle progression and in situ chromatin structure of L1210 cells" @default.
• W2015558270 cites W1503004123 @default.
• W2015558270 cites W1515078836 @default.
• W2015558270 cites W1525915127 @default.
• W2015558270 cites W1570767078 @default.
• W2015558270 cites W1579450536 @default.
• W2015558270 cites W1970107317 @default.
• W2015558270 cites W1983121846 @default.
• W2015558270 cites W1998006710 @default.
• W2015558270 cites W2001373008 @default.
• W2015558270 cites W2007873035 @default.
• W2015558270 cites W2010537904 @default.
• W2015558270 cites W2016332624 @default.
• W2015558270 cites W2025433329 @default.
• W2015558270 cites W2030037467 @default.
• W2015558270 cites W2032590819 @default.
• W2015558270 cites W2036545615 @default.
• W2015558270 cites W2044852019 @default.
• W2015558270 cites W2046571799 @default.
• W2015558270 cites W2049461710 @default.
• W2015558270 cites W2051237347 @default.
• W2015558270 cites W2052337911 @default.
• W2015558270 cites W2074003598 @default.
• W2015558270 cites W2106916231 @default.
• W2015558270 cites W2107270084 @default.
• W2015558270 cites W2125607576 @default.
• W2015558270 doi "https://doi.org/10.1016/0014-4827(81)90006-9" @default.
• W2015558270 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7308309" @default.
• W2015558270 hasPublicationYear "1981" @default.
• W2015558270 type Work @default.
• W2015558270 sameAs 2015558270 @default.
• W2015558270 citedByCount "76" @default.
• W2015558270 countsByYear W20155582702013 @default.
• W2015558270 countsByYear W20155582702015 @default.
• W2015558270 countsByYear W20155582702016 @default.
• W2015558270 countsByYear W20155582702017 @default.
• W2015558270 countsByYear W20155582702018 @default.
• W2015558270 countsByYear W20155582702021 @default.
• W2015558270 crossrefType "journal-article" @default.
• W2015558270 hasAuthorship W2015558270A5013493640 @default.
• W2015558270 hasAuthorship W2015558270A5033158298 @default.
• W2015558270 hasAuthorship W2015558270A5051515853 @default.
• W2015558270 hasAuthorship W2015558270A5084175495 @default.
• W2015558270 hasConcept C100544194 @default.
• W2015558270 hasConcept C12554922 @default.
• W2015558270 hasConcept C1491633281 @default.
• W2015558270 hasConcept C153911025 @default.
• W2015558270 hasConcept C2776979534 @default.
• W2015558270 hasConcept C29537977 @default.
• W2015558270 hasConcept C552990157 @default.
• W2015558270 hasConcept C55493867 @default.
• W2015558270 hasConcept C83640560 @default.
• W2015558270 hasConcept C86803240 @default.
• W2015558270 hasConcept C93304396 @default.
• W2015558270 hasConcept C95444343 @default.
• W2015558270 hasConceptScore W2015558270C100544194 @default.
• W2015558270 hasConceptScore W2015558270C12554922 @default.
• W2015558270 hasConceptScore W2015558270C1491633281 @default.
• W2015558270 hasConceptScore W2015558270C153911025 @default.
• W2015558270 hasConceptScore W2015558270C2776979534 @default.
• W2015558270 hasConceptScore W2015558270C29537977 @default.
• W2015558270 hasConceptScore W2015558270C552990157 @default.
• W2015558270 hasConceptScore W2015558270C55493867 @default.
• W2015558270 hasConceptScore W2015558270C83640560 @default.
• W2015558270 hasConceptScore W2015558270C86803240 @default.
• W2015558270 hasConceptScore W2015558270C93304396 @default.
• W2015558270 hasConceptScore W2015558270C95444343 @default.
• W2015558270 hasIssue "2" @default.
• W2015558270 hasLocation W20155582701 @default.
• W2015558270 hasLocation W20155582702 @default.
• W2015558270 hasOpenAccess W2015558270 @default.
• W2015558270 hasPrimaryLocation W20155582701 @default.
• W2015558270 hasRelatedWork W1544512904 @default.
• W2015558270 hasRelatedWork W2009772827 @default.
• W2015558270 hasRelatedWork W2045981880 @default.
• W2015558270 hasRelatedWork W2081626500 @default.
• W2015558270 hasRelatedWork W2083087496 @default.
• W2015558270 hasRelatedWork W2086802798 @default.
• W2015558270 hasRelatedWork W2097194453 @default.
• W2015558270 hasRelatedWork W2941764399 @default.
• W2015558270 hasRelatedWork W3126601605 @default.
• W2015558270 hasRelatedWork W4231069901 @default.
• W2015558270 hasVolume "136" @default.
• W2015558270 isParatext "false" @default.
• W2015558270 isRetracted "false" @default.
• W2015558270 magId "2015558270" @default.
• W2015558270 workType "article" @default.