FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015568979> ?p ?o ?g. }

• W2015568979 endingPage "49" @default.
• W2015568979 startingPage "42" @default.
• W2015568979 abstract "To achieve more information about growth and development of oocytes in teleost fish or concerning toxicity testing, it is necessary to develop adequate in vitro oocyte culture conditions. Herein, initial stages of zebrafish oocytes (I, primary; II, cortical; III, vitellogenic) were analyzed under serum-free medium conditions as gonadal fragments or as separated oocyte-follicle complexes. Two vital dye staining methods (MTT, trypan blue) were applied to assess mitochondrial activity and membrane integrity of the oocytes during 4 days, and compared to morphological alterations studied by transmission electron microscopy. Vital dye staining indicated reduced viability at day 4 for all stages in both in vitro culture methods. Additionally, the viability decreased significantly in gonadal fragments at day 2 for stages III (MTT, TB) and II (TB only). Signs of degradation at the ultrastructural level (vacuoles, disintegration of endoplasmic reticulum and detachment of follicular cell layers) appeared in gonadal fragments at day 4 for stages II and III, and in separated oocyte-follicle complexes both at day 4 for stages I-III, and at day 2 for stage III. In conclusion, zebrafish oocytes at stages I and II seemed viable for 2 days as separated oocyte-follicle complexes considering their mitochondrial activity, membrane integrity and ultrastructural morphology. Cultured as gonadal fragments, the majority of analyses indicated similar results for stages I and II oocytes. In contrast, stage III oocytes seemed viable for not longer than 24 h. Results should be taken into consideration for the experimental design of in vitro assays using teleost fish oocytes." @default.
• W2015568979 created "2016-06-24" @default.
• W2015568979 creator A5041533307 @default.
• W2015568979 creator A5043545492 @default.
• W2015568979 creator A5056208095 @default.
• W2015568979 creator A5061581521 @default.
• W2015568979 creator A5078596969 @default.
• W2015568979 creator A5080609495 @default.
• W2015568979 date "2013-10-22" @default.
• W2015568979 modified "2023-09-22" @default.
• W2015568979 title "Viability analysis of oocyte–follicle complexes and gonadal fragments of zebrafish as baseline for toxicity testing" @default.
• W2015568979 cites W1554390204 @default.
• W2015568979 cites W1849819493 @default.
• W2015568979 cites W1971438031 @default.
• W2015568979 cites W1979862433 @default.
• W2015568979 cites W1992818604 @default.
• W2015568979 cites W2005879317 @default.
• W2015568979 cites W2014168082 @default.
• W2015568979 cites W2027454264 @default.
• W2015568979 cites W2030222301 @default.
• W2015568979 cites W2041788568 @default.
• W2015568979 cites W2051718609 @default.
• W2015568979 cites W2068591187 @default.
• W2015568979 cites W2072439295 @default.
• W2015568979 cites W2073540391 @default.
• W2015568979 cites W2078602147 @default.
• W2015568979 cites W2087176961 @default.
• W2015568979 cites W2095247369 @default.
• W2015568979 cites W2095841223 @default.
• W2015568979 cites W2098586878 @default.
• W2015568979 cites W2105444449 @default.
• W2015568979 cites W2107382554 @default.
• W2015568979 cites W2110825908 @default.
• W2015568979 cites W2118457069 @default.
• W2015568979 cites W2127911529 @default.
• W2015568979 cites W2133007496 @default.
• W2015568979 cites W2151189998 @default.
• W2015568979 cites W2153267327 @default.
• W2015568979 cites W2154035365 @default.
• W2015568979 cites W2170403716 @default.
• W2015568979 cites W3759375 @default.
• W2015568979 doi "https://doi.org/10.3109/15376516.2013.846952" @default.
• W2015568979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24053232" @default.
• W2015568979 hasPublicationYear "2013" @default.
• W2015568979 type Work @default.
• W2015568979 sameAs 2015568979 @default.
• W2015568979 citedByCount "5" @default.
• W2015568979 countsByYear W20155689792015 @default.
• W2015568979 countsByYear W20155689792016 @default.
• W2015568979 countsByYear W20155689792018 @default.
• W2015568979 countsByYear W20155689792021 @default.
• W2015568979 crossrefType "journal-article" @default.
• W2015568979 hasAuthorship W2015568979A5041533307 @default.
• W2015568979 hasAuthorship W2015568979A5043545492 @default.
• W2015568979 hasAuthorship W2015568979A5056208095 @default.
• W2015568979 hasAuthorship W2015568979A5061581521 @default.
• W2015568979 hasAuthorship W2015568979A5078596969 @default.
• W2015568979 hasAuthorship W2015568979A5080609495 @default.
• W2015568979 hasConcept C102570824 @default.
• W2015568979 hasConcept C104317684 @default.
• W2015568979 hasConcept C105702510 @default.
• W2015568979 hasConcept C126322002 @default.
• W2015568979 hasConcept C134018914 @default.
• W2015568979 hasConcept C158617107 @default.
• W2015568979 hasConcept C16685009 @default.
• W2015568979 hasConcept C187785154 @default.
• W2015568979 hasConcept C196843134 @default.
• W2015568979 hasConcept C202751555 @default.
• W2015568979 hasConcept C2775966511 @default.
• W2015568979 hasConcept C2776690073 @default.
• W2015568979 hasConcept C2776878037 @default.
• W2015568979 hasConcept C2780070996 @default.
• W2015568979 hasConcept C2780536345 @default.
• W2015568979 hasConcept C29730261 @default.
• W2015568979 hasConcept C53227056 @default.
• W2015568979 hasConcept C5512455 @default.
• W2015568979 hasConcept C55493867 @default.
• W2015568979 hasConcept C56366318 @default.
• W2015568979 hasConcept C71924100 @default.
• W2015568979 hasConcept C86803240 @default.
• W2015568979 hasConcept C87073359 @default.
• W2015568979 hasConcept C87555872 @default.
• W2015568979 hasConcept C95444343 @default.
• W2015568979 hasConceptScore W2015568979C102570824 @default.
• W2015568979 hasConceptScore W2015568979C104317684 @default.
• W2015568979 hasConceptScore W2015568979C105702510 @default.
• W2015568979 hasConceptScore W2015568979C126322002 @default.
• W2015568979 hasConceptScore W2015568979C134018914 @default.
• W2015568979 hasConceptScore W2015568979C158617107 @default.
• W2015568979 hasConceptScore W2015568979C16685009 @default.
• W2015568979 hasConceptScore W2015568979C187785154 @default.
• W2015568979 hasConceptScore W2015568979C196843134 @default.
• W2015568979 hasConceptScore W2015568979C202751555 @default.
• W2015568979 hasConceptScore W2015568979C2775966511 @default.
• W2015568979 hasConceptScore W2015568979C2776690073 @default.
• W2015568979 hasConceptScore W2015568979C2776878037 @default.
• W2015568979 hasConceptScore W2015568979C2780070996 @default.
• W2015568979 hasConceptScore W2015568979C2780536345 @default.

• next