Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015581772> ?p ?o ?g. }
- W2015581772 endingPage "425" @default.
- W2015581772 startingPage "419" @default.
- W2015581772 abstract "Chikungunya has emerged as one of the most important arboviral infection of global significance. Expansion of Chikungunya virus endemic areas can be ascribed to naive population, increasing vector population and adaptability of virus to new vector. In this study, a SYBR Green I based quantitative RT-PCR assay was developed. The assay was found to be 10-fold more sensitive than conventional RT-PCR and no cross reactivity was observed with related alphaviruses and flaviviruses. The detection efficiency of the assay was impervious to mosquitoes of different pool sizes. Vector surveillance has resulted in detection of CHIKV RNA in Aedes aegypti, confirming its vectorial potential for CHIKV in northern India. The assessment of the assay was further carried out by studying the competence of Indian Ae. aegypti for CHIKV, which revealed 100% infection rate and dissemination rate with 60% transmission rate. The replication kinetics of CHIKV in different anatomical sites of Ae. aegypti revealed highest titre at day 6 post infection in midgut and at day 10 post infection in saliva, legs and wings. The implementation of the assay in detecting lower viral load makes it a remarkable tool for surveillance of virus activity in mosquitoes." @default.
- W2015581772 created "2016-06-24" @default.
- W2015581772 creator A5030820229 @default.
- W2015581772 creator A5033743247 @default.
- W2015581772 creator A5039793037 @default.
- W2015581772 creator A5040776132 @default.
- W2015581772 creator A5040998536 @default.
- W2015581772 creator A5052925514 @default.
- W2015581772 creator A5077914585 @default.
- W2015581772 creator A5083702024 @default.
- W2015581772 creator A5090134740 @default.
- W2015581772 date "2013-11-01" @default.
- W2015581772 modified "2023-09-26" @default.
- W2015581772 title "Application of real-time RT-PCR in vector surveillance and assessment of replication kinetics of an emerging novel ECSA genotype of Chikungunya virus in Aedes aegypti" @default.
- W2015581772 cites W1971242896 @default.
- W2015581772 cites W1984332517 @default.
- W2015581772 cites W1999780961 @default.
- W2015581772 cites W2000241775 @default.
- W2015581772 cites W2004189606 @default.
- W2015581772 cites W2005808396 @default.
- W2015581772 cites W2012994493 @default.
- W2015581772 cites W2021045663 @default.
- W2015581772 cites W2026422745 @default.
- W2015581772 cites W2034377840 @default.
- W2015581772 cites W2038180816 @default.
- W2015581772 cites W2047983645 @default.
- W2015581772 cites W2051304065 @default.
- W2015581772 cites W2054758742 @default.
- W2015581772 cites W2062504884 @default.
- W2015581772 cites W2070568063 @default.
- W2015581772 cites W2079953254 @default.
- W2015581772 cites W2081858758 @default.
- W2015581772 cites W2098987638 @default.
- W2015581772 cites W2103265014 @default.
- W2015581772 cites W2119867242 @default.
- W2015581772 cites W2125616127 @default.
- W2015581772 cites W2127043657 @default.
- W2015581772 cites W2137750232 @default.
- W2015581772 cites W2146387016 @default.
- W2015581772 cites W2146562448 @default.
- W2015581772 cites W2156559833 @default.
- W2015581772 cites W2164071075 @default.
- W2015581772 cites W2168695613 @default.
- W2015581772 cites W4320800933 @default.
- W2015581772 doi "https://doi.org/10.1016/j.jviromet.2013.07.004" @default.
- W2015581772 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23850695" @default.
- W2015581772 hasPublicationYear "2013" @default.
- W2015581772 type Work @default.
- W2015581772 sameAs 2015581772 @default.
- W2015581772 citedByCount "17" @default.
- W2015581772 countsByYear W20155817722013 @default.
- W2015581772 countsByYear W20155817722014 @default.
- W2015581772 countsByYear W20155817722015 @default.
- W2015581772 countsByYear W20155817722016 @default.
- W2015581772 countsByYear W20155817722017 @default.
- W2015581772 countsByYear W20155817722019 @default.
- W2015581772 countsByYear W20155817722020 @default.
- W2015581772 countsByYear W20155817722021 @default.
- W2015581772 countsByYear W20155817722022 @default.
- W2015581772 crossrefType "journal-article" @default.
- W2015581772 hasAuthorship W2015581772A5030820229 @default.
- W2015581772 hasAuthorship W2015581772A5033743247 @default.
- W2015581772 hasAuthorship W2015581772A5039793037 @default.
- W2015581772 hasAuthorship W2015581772A5040776132 @default.
- W2015581772 hasAuthorship W2015581772A5040998536 @default.
- W2015581772 hasAuthorship W2015581772A5052925514 @default.
- W2015581772 hasAuthorship W2015581772A5077914585 @default.
- W2015581772 hasAuthorship W2015581772A5083702024 @default.
- W2015581772 hasAuthorship W2015581772A5090134740 @default.
- W2015581772 hasConcept C104317684 @default.
- W2015581772 hasConcept C144024400 @default.
- W2015581772 hasConcept C149923435 @default.
- W2015581772 hasConcept C159047783 @default.
- W2015581772 hasConcept C173758957 @default.
- W2015581772 hasConcept C18903297 @default.
- W2015581772 hasConcept C2522874641 @default.
- W2015581772 hasConcept C2777775583 @default.
- W2015581772 hasConcept C2778116200 @default.
- W2015581772 hasConcept C2779755945 @default.
- W2015581772 hasConcept C2780688631 @default.
- W2015581772 hasConcept C2781273456 @default.
- W2015581772 hasConcept C2908647359 @default.
- W2015581772 hasConcept C40767141 @default.
- W2015581772 hasConcept C48023723 @default.
- W2015581772 hasConcept C533803919 @default.
- W2015581772 hasConcept C54355233 @default.
- W2015581772 hasConcept C86803240 @default.
- W2015581772 hasConcept C92087593 @default.
- W2015581772 hasConceptScore W2015581772C104317684 @default.
- W2015581772 hasConceptScore W2015581772C144024400 @default.
- W2015581772 hasConceptScore W2015581772C149923435 @default.
- W2015581772 hasConceptScore W2015581772C159047783 @default.
- W2015581772 hasConceptScore W2015581772C173758957 @default.
- W2015581772 hasConceptScore W2015581772C18903297 @default.
- W2015581772 hasConceptScore W2015581772C2522874641 @default.
- W2015581772 hasConceptScore W2015581772C2777775583 @default.
- W2015581772 hasConceptScore W2015581772C2778116200 @default.
- W2015581772 hasConceptScore W2015581772C2779755945 @default.