Matches in SemOpenAlex for { <https://semopenalex.org/work/W201558303> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W201558303 endingPage "15" @default.
- W201558303 startingPage "109" @default.
- W201558303 abstract "Mixed lymphocyte reaction (MLC) of T-lymphocytes with dendritic cells is one of the basic tools for studying of immune reaction mechanisms. This work describes a new method of evaluation of allogeneic and autologous MLC by flow cytometry and differential gating. This method is based on fixed time acquisition of events and their sorting according to their forward and side scatter properties. Differential gating distinguishes populations of all living cells (R1), living non-proliferating cells (R2) and dead/apoptotic cells (R4). Addition of fluorescence microspheres (R3) enables the calculation of absolute number of cells in a given sample volume. Using the method of 7-AAD positive cells project into the exclusion, we have shown that approximately 90% of 7-AAD R4 gate. Moreover, there was an excellent correlation between the R1: R2 ratio and the percentage of cells positive for activation/proliferation marker CD71. The method of differential gating in its basic version does not require use of any fluorescent antibody and therefore is suitable for rapid screening of large number of samples at reasonable cost and without the use of radiolabeled nucleotides." @default.
- W201558303 created "2016-06-24" @default.
- W201558303 creator A5021513448 @default.
- W201558303 creator A5047395452 @default.
- W201558303 creator A5076581809 @default.
- W201558303 creator A5089171072 @default.
- W201558303 date "2005-08-01" @default.
- W201558303 modified "2023-09-23" @default.
- W201558303 title "[Flow cytometric differential gating strategy for evaluation of T-lymphocyte survival and proliferation in mixed lymphocyte reaction with dendritic cells]." @default.
- W201558303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16173521" @default.
- W201558303 hasPublicationYear "2005" @default.
- W201558303 type Work @default.
- W201558303 sameAs 201558303 @default.
- W201558303 citedByCount "1" @default.
- W201558303 crossrefType "journal-article" @default.
- W201558303 hasAuthorship W201558303A5021513448 @default.
- W201558303 hasAuthorship W201558303A5047395452 @default.
- W201558303 hasAuthorship W201558303A5076581809 @default.
- W201558303 hasAuthorship W201558303A5089171072 @default.
- W201558303 hasConcept C12554922 @default.
- W201558303 hasConcept C185592680 @default.
- W201558303 hasConcept C194544171 @default.
- W201558303 hasConcept C203014093 @default.
- W201558303 hasConcept C2777761686 @default.
- W201558303 hasConcept C2778630268 @default.
- W201558303 hasConcept C2992881475 @default.
- W201558303 hasConcept C553184892 @default.
- W201558303 hasConcept C86803240 @default.
- W201558303 hasConcept C8891405 @default.
- W201558303 hasConcept C95444343 @default.
- W201558303 hasConceptScore W201558303C12554922 @default.
- W201558303 hasConceptScore W201558303C185592680 @default.
- W201558303 hasConceptScore W201558303C194544171 @default.
- W201558303 hasConceptScore W201558303C203014093 @default.
- W201558303 hasConceptScore W201558303C2777761686 @default.
- W201558303 hasConceptScore W201558303C2778630268 @default.
- W201558303 hasConceptScore W201558303C2992881475 @default.
- W201558303 hasConceptScore W201558303C553184892 @default.
- W201558303 hasConceptScore W201558303C86803240 @default.
- W201558303 hasConceptScore W201558303C8891405 @default.
- W201558303 hasConceptScore W201558303C95444343 @default.
- W201558303 hasIssue "3" @default.
- W201558303 hasLocation W2015583031 @default.
- W201558303 hasOpenAccess W201558303 @default.
- W201558303 hasPrimaryLocation W2015583031 @default.
- W201558303 hasRelatedWork W1594577646 @default.
- W201558303 hasRelatedWork W1970331628 @default.
- W201558303 hasRelatedWork W2012218854 @default.
- W201558303 hasRelatedWork W2020930943 @default.
- W201558303 hasRelatedWork W2021486585 @default.
- W201558303 hasRelatedWork W2027203361 @default.
- W201558303 hasRelatedWork W2056230941 @default.
- W201558303 hasRelatedWork W2062282919 @default.
- W201558303 hasRelatedWork W2080601632 @default.
- W201558303 hasRelatedWork W2090946470 @default.
- W201558303 hasRelatedWork W2113998585 @default.
- W201558303 hasRelatedWork W2124930007 @default.
- W201558303 hasRelatedWork W2153524211 @default.
- W201558303 hasRelatedWork W2376761853 @default.
- W201558303 hasRelatedWork W2394443763 @default.
- W201558303 hasRelatedWork W2770659346 @default.
- W201558303 hasRelatedWork W3026911797 @default.
- W201558303 hasRelatedWork W3045322635 @default.
- W201558303 hasRelatedWork W3202034003 @default.
- W201558303 hasRelatedWork W783002247 @default.
- W201558303 hasVolume "54" @default.
- W201558303 isParatext "false" @default.
- W201558303 isRetracted "false" @default.
- W201558303 magId "201558303" @default.
- W201558303 workType "article" @default.