Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015600463> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2015600463 endingPage "353" @default.
- W2015600463 startingPage "347" @default.
- W2015600463 abstract "Calmodulin-dependent protein kinase II was purified to apparent homogeneity with a high yield from the total calmodulin-binding protein fraction of bovine cardiac muscle in a single step by gel filtration column chromatography. This procedure is simple and suitable for adaptation to large scale preparations. The purified calmodulin-dependent protein kinase has a specific enzymic activity of 2.4 mumol/min/mg when mixed histone was used as a substrate. The preparation of enzyme appears to be homogeneous when examined by SDS-PAGE. The molecular weight of the enzyme was determined to be 570 kDa by gel filtration. SDS-PAGE of the enzyme subunit showed a single protein band with an apparent molecular weight of 56 kDa. These results suggest that the calmodulin-dependent protein kinase II from bovine heart is composed of 10 identical subunits. Anti-peptide antibody raised against multifunctional calmodulin-dependent protein kinase II from rat brain showed a single immunoreactive band of 56 kDa on Western blot. These results suggested that bovine cardiac muscle calmodulin-dependent protein kinase could resemble the brain isozyme. Calmodulin-dependent protein kinase II undergoes autophosphorylation with a maximal incorporation of 1 mol of phosphate per mol of the subunit of the enzyme and the autophosphorylated enzyme remains active in the absence of Ca2+ and calmodulin. The concentration of Ca2+ required for the activation of calmodulin-dependent protein kinase II depends on the level of calmodulin in the reaction." @default.
- W2015600463 created "2016-06-24" @default.
- W2015600463 creator A5003046543 @default.
- W2015600463 creator A5050620780 @default.
- W2015600463 creator A5059518752 @default.
- W2015600463 date "1996-10-01" @default.
- W2015600463 modified "2023-10-14" @default.
- W2015600463 title "Calmodulinwdependent protein kinase II from bovine cardiac muscle: purification and differential activation by calcium" @default.
- W2015600463 cites W1480101062 @default.
- W2015600463 cites W1514339680 @default.
- W2015600463 cites W1526920045 @default.
- W2015600463 cites W1540403838 @default.
- W2015600463 cites W1540771766 @default.
- W2015600463 cites W1560918162 @default.
- W2015600463 cites W1597249542 @default.
- W2015600463 cites W1607322826 @default.
- W2015600463 cites W1634129492 @default.
- W2015600463 cites W1642193049 @default.
- W2015600463 cites W1841822105 @default.
- W2015600463 cites W1967479968 @default.
- W2015600463 cites W1986296266 @default.
- W2015600463 cites W1986656675 @default.
- W2015600463 cites W1993282564 @default.
- W2015600463 cites W2033942553 @default.
- W2015600463 cites W2043312032 @default.
- W2015600463 cites W2045632212 @default.
- W2015600463 cites W2049548434 @default.
- W2015600463 cites W2091101609 @default.
- W2015600463 cites W2100837269 @default.
- W2015600463 cites W2149714663 @default.
- W2015600463 cites W2160437891 @default.
- W2015600463 cites W2180781622 @default.
- W2015600463 cites W2335286373 @default.
- W2015600463 cites W2340275040 @default.
- W2015600463 cites W4211204946 @default.
- W2015600463 cites W4247303250 @default.
- W2015600463 cites W4249424055 @default.
- W2015600463 cites W4293247451 @default.
- W2015600463 doi "https://doi.org/10.1016/s0143-4160(96)90040-8" @default.
- W2015600463 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8939354" @default.
- W2015600463 hasPublicationYear "1996" @default.
- W2015600463 type Work @default.
- W2015600463 sameAs 2015600463 @default.
- W2015600463 citedByCount "4" @default.
- W2015600463 countsByYear W20156004632017 @default.
- W2015600463 countsByYear W20156004632022 @default.
- W2015600463 crossrefType "journal-article" @default.
- W2015600463 hasAuthorship W2015600463A5003046543 @default.
- W2015600463 hasAuthorship W2015600463A5050620780 @default.
- W2015600463 hasAuthorship W2015600463A5059518752 @default.
- W2015600463 hasConcept C104292427 @default.
- W2015600463 hasConcept C104317684 @default.
- W2015600463 hasConcept C150109051 @default.
- W2015600463 hasConcept C153911025 @default.
- W2015600463 hasConcept C181199279 @default.
- W2015600463 hasConcept C185592680 @default.
- W2015600463 hasConcept C193363816 @default.
- W2015600463 hasConcept C29688787 @default.
- W2015600463 hasConcept C55493867 @default.
- W2015600463 hasConcept C86803240 @default.
- W2015600463 hasConcept C97029542 @default.
- W2015600463 hasConceptScore W2015600463C104292427 @default.
- W2015600463 hasConceptScore W2015600463C104317684 @default.
- W2015600463 hasConceptScore W2015600463C150109051 @default.
- W2015600463 hasConceptScore W2015600463C153911025 @default.
- W2015600463 hasConceptScore W2015600463C181199279 @default.
- W2015600463 hasConceptScore W2015600463C185592680 @default.
- W2015600463 hasConceptScore W2015600463C193363816 @default.
- W2015600463 hasConceptScore W2015600463C29688787 @default.
- W2015600463 hasConceptScore W2015600463C55493867 @default.
- W2015600463 hasConceptScore W2015600463C86803240 @default.
- W2015600463 hasConceptScore W2015600463C97029542 @default.
- W2015600463 hasIssue "4" @default.
- W2015600463 hasLocation W20156004631 @default.
- W2015600463 hasLocation W20156004632 @default.
- W2015600463 hasOpenAccess W2015600463 @default.
- W2015600463 hasPrimaryLocation W20156004631 @default.
- W2015600463 hasRelatedWork W1418314897 @default.
- W2015600463 hasRelatedWork W1562207750 @default.
- W2015600463 hasRelatedWork W1581932487 @default.
- W2015600463 hasRelatedWork W1989024791 @default.
- W2015600463 hasRelatedWork W1992394481 @default.
- W2015600463 hasRelatedWork W1998678953 @default.
- W2015600463 hasRelatedWork W2011962611 @default.
- W2015600463 hasRelatedWork W2015600463 @default.
- W2015600463 hasRelatedWork W2085148012 @default.
- W2015600463 hasRelatedWork W2419260826 @default.
- W2015600463 hasVolume "20" @default.
- W2015600463 isParatext "false" @default.
- W2015600463 isRetracted "false" @default.
- W2015600463 magId "2015600463" @default.
- W2015600463 workType "article" @default.