Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015612833> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2015612833 endingPage "602a" @default.
- W2015612833 startingPage "602a" @default.
- W2015612833 abstract "Phospholamban (PLN) is a 52-residue membrane protein that regulates the cardiac sarcoplasmic reticulum Ca2+ATPase (SERCA). PLN is in equilibrium between monomeric and pentameric species. The monomer binds to and decreases the rate of Ca2+ transport of SERCA. This inhibitory action is relieved upon PLN phosphorylation at Serine-16 and Threonine-17. Here, we present the structural dynamics of fully phosphorylated pentameric PLN by a combination of solution and solid-state NMR spectroscopy. SDS-PAGE analysis revealed a higher thermal stability of the phosphorylated pentameric PLN (at Ser-16, Thr-17 and both) with respect to the unphosphorylated protein when reconstituted in different lipids and detergents.Conventional T1, T2, and heteronuclear steady state NOEs experiments were used to probe fast (ps-ns) dynamics of the amide backbone upon phosphorylation at Ser-16, revealing a significant increase of the dynamics in this time scale. Slower (µs-ms) dynamics were probed by measuring rotating frame R(1rho) and R(2rho) dispersion curves using a new adiabatic irradiation scheme.The structural and topological effects of phosphorylation in pentameric PLN were investigated using solid-state NMR experiments on mechanically and magnetically oriented lipid systems. For the mechanically oriented samples, phosphorylated PLN was reconstituted in DOPC/DOPE lipid bilayers and uniaxially aligned between glass plates. For magnetically oriented samples, the protein was reconstituted in DMPC/Triton X-100 bicelles. Two-dimensional PISEMA and SAMPI4 experiments were used to determine the topology of PLN in lipid membranes. Chemical shifts anisotropy and dipolar coupling values were also used in combination with solution NMR restraints to determine a preliminary high-resolution structure of fully phosphorylated pentameric PLN.These studies reveal residue-by-residue details of the pentameric PLN structure and topology upon phosphorylation." @default.
- W2015612833 created "2016-06-24" @default.
- W2015612833 creator A5008220934 @default.
- W2015612833 creator A5009664013 @default.
- W2015612833 creator A5044049726 @default.
- W2015612833 creator A5075025150 @default.
- W2015612833 date "2011-02-01" @default.
- W2015612833 modified "2023-09-29" @default.
- W2015612833 title "Structural Dynamics of Phosphorylated Pentameric Phospholamban in Lipid Membranes using a Combination of Solution and Solid-State NMR Spectroscopy" @default.
- W2015612833 doi "https://doi.org/10.1016/j.bpj.2010.12.3466" @default.
- W2015612833 hasPublicationYear "2011" @default.
- W2015612833 type Work @default.
- W2015612833 sameAs 2015612833 @default.
- W2015612833 citedByCount "0" @default.
- W2015612833 crossrefType "journal-article" @default.
- W2015612833 hasAuthorship W2015612833A5008220934 @default.
- W2015612833 hasAuthorship W2015612833A5009664013 @default.
- W2015612833 hasAuthorship W2015612833A5044049726 @default.
- W2015612833 hasAuthorship W2015612833A5075025150 @default.
- W2015612833 hasBestOaLocation W20156128331 @default.
- W2015612833 hasConcept C11960822 @default.
- W2015612833 hasConcept C121332964 @default.
- W2015612833 hasConcept C12554922 @default.
- W2015612833 hasConcept C153202636 @default.
- W2015612833 hasConcept C17137333 @default.
- W2015612833 hasConcept C181199279 @default.
- W2015612833 hasConcept C185592680 @default.
- W2015612833 hasConcept C23265538 @default.
- W2015612833 hasConcept C2781414619 @default.
- W2015612833 hasConcept C39944091 @default.
- W2015612833 hasConcept C41625074 @default.
- W2015612833 hasConcept C46141821 @default.
- W2015612833 hasConcept C55493867 @default.
- W2015612833 hasConcept C66974803 @default.
- W2015612833 hasConcept C71240020 @default.
- W2015612833 hasConcept C8010536 @default.
- W2015612833 hasConcept C86803240 @default.
- W2015612833 hasConceptScore W2015612833C11960822 @default.
- W2015612833 hasConceptScore W2015612833C121332964 @default.
- W2015612833 hasConceptScore W2015612833C12554922 @default.
- W2015612833 hasConceptScore W2015612833C153202636 @default.
- W2015612833 hasConceptScore W2015612833C17137333 @default.
- W2015612833 hasConceptScore W2015612833C181199279 @default.
- W2015612833 hasConceptScore W2015612833C185592680 @default.
- W2015612833 hasConceptScore W2015612833C23265538 @default.
- W2015612833 hasConceptScore W2015612833C2781414619 @default.
- W2015612833 hasConceptScore W2015612833C39944091 @default.
- W2015612833 hasConceptScore W2015612833C41625074 @default.
- W2015612833 hasConceptScore W2015612833C46141821 @default.
- W2015612833 hasConceptScore W2015612833C55493867 @default.
- W2015612833 hasConceptScore W2015612833C66974803 @default.
- W2015612833 hasConceptScore W2015612833C71240020 @default.
- W2015612833 hasConceptScore W2015612833C8010536 @default.
- W2015612833 hasConceptScore W2015612833C86803240 @default.
- W2015612833 hasIssue "3" @default.
- W2015612833 hasLocation W20156128331 @default.
- W2015612833 hasOpenAccess W2015612833 @default.
- W2015612833 hasPrimaryLocation W20156128331 @default.
- W2015612833 hasRelatedWork W1963980612 @default.
- W2015612833 hasRelatedWork W1976905220 @default.
- W2015612833 hasRelatedWork W1986635292 @default.
- W2015612833 hasRelatedWork W2004124155 @default.
- W2015612833 hasRelatedWork W2009514212 @default.
- W2015612833 hasRelatedWork W2016870805 @default.
- W2015612833 hasRelatedWork W2019996679 @default.
- W2015612833 hasRelatedWork W2027277904 @default.
- W2015612833 hasRelatedWork W2042372200 @default.
- W2015612833 hasRelatedWork W2085647989 @default.
- W2015612833 hasRelatedWork W2103504451 @default.
- W2015612833 hasRelatedWork W2115844655 @default.
- W2015612833 hasRelatedWork W2116475953 @default.
- W2015612833 hasRelatedWork W2118719530 @default.
- W2015612833 hasRelatedWork W2124984795 @default.
- W2015612833 hasRelatedWork W2159465090 @default.
- W2015612833 hasRelatedWork W2328251141 @default.
- W2015612833 hasRelatedWork W2334569365 @default.
- W2015612833 hasRelatedWork W3018812826 @default.
- W2015612833 hasRelatedWork W3022176737 @default.
- W2015612833 hasVolume "100" @default.
- W2015612833 isParatext "false" @default.
- W2015612833 isRetracted "false" @default.
- W2015612833 magId "2015612833" @default.
- W2015612833 workType "article" @default.