Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015619236> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2015619236 endingPage "6511" @default.
- W2015619236 startingPage "6507" @default.
- W2015619236 abstract "The plasma membrane from the human tumor astrocytoma contains an active acid phosphatase activity based on hydrolysis of p-nitrophenyl phosphate. Other acid phosphatase substrates--beta-glycerophosphate, O-phosphorylcholine, and 5'-AMP--are not hydrolyzed significantly. The phosphatase activity is tartrate insensitive and is stimulated by Triton X-100 and EDTA. Of the three known phosphoamino acids, only free O-phosphotyrosine is hydrolyzed by the membrane phosphatase activity. Other acid phosphatases tested from potato, wheat germ, milk, and bovine prostate did not show this degree of specificity. The plasma membrane activity also dephosphorylated phosphotyrosine histone at a much greater rate than did the other acid phosphatases. pH profiles for free O-phosphotyrosine and phosphotyrosine histone showed a shift toward physiological pH, indicating possible physiological significance. Phosphotyrosine histone dephosphorylation activity was nearly 10 times greater than that seen for phosphoserine histone dephosphorylation, and Km values were much lower for phosphotyrosine histone dephosphorylation (0.5 microM vs. 10 microM). Fluoride and zinc significantly inhibited phosphoserine histone dephosphorylation. Vanadate, on the other hand, was a potent inhibitor of phosphotyrosine histone dephosphorylation (50% inhibition at 0.5 microM) but not of phosphoserine histone. ATP stimulated phosphotyrosine histone dephosphorylation (160-250%) but inhibited phosphoserine histone dephosphorylation (95%). These results suggest the existence of a highly specific phosphotyrosine protein phosphatase activity associated with the plasma membrane of human astrocytoma." @default.
- W2015619236 created "2016-06-24" @default.
- W2015619236 creator A5084890905 @default.
- W2015619236 creator A5087950597 @default.
- W2015619236 date "1982-11-01" @default.
- W2015619236 modified "2023-09-25" @default.
- W2015619236 title "An acid phosphatase in the plasma membranes of human astrocytoma showing marked specificity toward phosphotyrosine protein." @default.
- W2015619236 cites W126219881 @default.
- W2015619236 cites W148345225 @default.
- W2015619236 cites W1505870408 @default.
- W2015619236 cites W1522098040 @default.
- W2015619236 cites W1524333424 @default.
- W2015619236 cites W1544586151 @default.
- W2015619236 cites W1576399086 @default.
- W2015619236 cites W1579064499 @default.
- W2015619236 cites W1599070783 @default.
- W2015619236 cites W193459211 @default.
- W2015619236 cites W1995729295 @default.
- W2015619236 cites W2011147958 @default.
- W2015619236 cites W2018275786 @default.
- W2015619236 cites W2026748923 @default.
- W2015619236 cites W2049583947 @default.
- W2015619236 cites W2056770304 @default.
- W2015619236 cites W2071273962 @default.
- W2015619236 cites W2120012514 @default.
- W2015619236 cites W2211924732 @default.
- W2015619236 cites W2267588421 @default.
- W2015619236 cites W2412508622 @default.
- W2015619236 doi "https://doi.org/10.1073/pnas.79.21.6507" @default.
- W2015619236 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/347156" @default.
- W2015619236 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6183663" @default.
- W2015619236 hasPublicationYear "1982" @default.
- W2015619236 type Work @default.
- W2015619236 sameAs 2015619236 @default.
- W2015619236 citedByCount "122" @default.
- W2015619236 countsByYear W20156192362017 @default.
- W2015619236 countsByYear W20156192362022 @default.
- W2015619236 crossrefType "journal-article" @default.
- W2015619236 hasAuthorship W2015619236A5084890905 @default.
- W2015619236 hasAuthorship W2015619236A5087950597 @default.
- W2015619236 hasBestOaLocation W20156192361 @default.
- W2015619236 hasConcept C11960822 @default.
- W2015619236 hasConcept C153911025 @default.
- W2015619236 hasConcept C178666793 @default.
- W2015619236 hasConcept C181912034 @default.
- W2015619236 hasConcept C185592680 @default.
- W2015619236 hasConcept C2776414213 @default.
- W2015619236 hasConcept C2780307904 @default.
- W2015619236 hasConcept C389152 @default.
- W2015619236 hasConcept C55493867 @default.
- W2015619236 hasConcept C86803240 @default.
- W2015619236 hasConcept C97920573 @default.
- W2015619236 hasConceptScore W2015619236C11960822 @default.
- W2015619236 hasConceptScore W2015619236C153911025 @default.
- W2015619236 hasConceptScore W2015619236C178666793 @default.
- W2015619236 hasConceptScore W2015619236C181912034 @default.
- W2015619236 hasConceptScore W2015619236C185592680 @default.
- W2015619236 hasConceptScore W2015619236C2776414213 @default.
- W2015619236 hasConceptScore W2015619236C2780307904 @default.
- W2015619236 hasConceptScore W2015619236C389152 @default.
- W2015619236 hasConceptScore W2015619236C55493867 @default.
- W2015619236 hasConceptScore W2015619236C86803240 @default.
- W2015619236 hasConceptScore W2015619236C97920573 @default.
- W2015619236 hasIssue "21" @default.
- W2015619236 hasLocation W20156192361 @default.
- W2015619236 hasLocation W20156192362 @default.
- W2015619236 hasLocation W20156192363 @default.
- W2015619236 hasLocation W20156192364 @default.
- W2015619236 hasOpenAccess W2015619236 @default.
- W2015619236 hasPrimaryLocation W20156192361 @default.
- W2015619236 hasRelatedWork W1477071538 @default.
- W2015619236 hasRelatedWork W1970148057 @default.
- W2015619236 hasRelatedWork W1985633388 @default.
- W2015619236 hasRelatedWork W2015862623 @default.
- W2015619236 hasRelatedWork W2054888896 @default.
- W2015619236 hasRelatedWork W2071548379 @default.
- W2015619236 hasRelatedWork W2109572947 @default.
- W2015619236 hasRelatedWork W2334397226 @default.
- W2015619236 hasRelatedWork W2464145031 @default.
- W2015619236 hasRelatedWork W2507843907 @default.
- W2015619236 hasVolume "79" @default.
- W2015619236 isParatext "false" @default.
- W2015619236 isRetracted "false" @default.
- W2015619236 magId "2015619236" @default.
- W2015619236 workType "article" @default.