Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015624242> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2015624242 endingPage "155" @default.
- W2015624242 startingPage "150" @default.
- W2015624242 abstract "The reaction of trypsin with activated monomethoxypoly(ethylene glycol) with various molecular masses led to the development of a series of poly(ethylene glycol)-modified trypsins (PEG-trypsins). On determining the catalytic properties of PEG-trypsin using N-benzoyl-L-arginine p-nitroanilide as a substrate, a three- to fourfold increase in the maximal velocity of hydrolysis was found to occur, whatever the size of the PEG moiety used. PEG-trypsin with higher molecular mass moieties showed lower Michaelis constant values. The activation of trypsin was neither reversed by nucleophiles such as hydroxylamine, nor prevented when modification was carried out in the presence of benzamidine or in the presence of the polypeptidic soybean trypsin inhibitor. Chemical modification of about 80% of the free amino groups with PEG chains significantly improved the resistance to heat and detergents. This might result from the formation of a highly hydrogen-bonded structure around the enzyme." @default.
- W2015624242 created "2016-06-24" @default.
- W2015624242 creator A5030768615 @default.
- W2015624242 creator A5037902379 @default.
- W2015624242 date "1992-02-01" @default.
- W2015624242 modified "2023-10-14" @default.
- W2015624242 title "Increased activity and stability of poly(ethylene glycol)-modified trypsin" @default.
- W2015624242 cites W1490299254 @default.
- W2015624242 cites W1966497279 @default.
- W2015624242 cites W1968114644 @default.
- W2015624242 cites W1980683696 @default.
- W2015624242 cites W2006208256 @default.
- W2015624242 cites W2018215401 @default.
- W2015624242 cites W2021240740 @default.
- W2015624242 cites W2022537484 @default.
- W2015624242 cites W2029176174 @default.
- W2015624242 cites W2033689719 @default.
- W2015624242 cites W2070776218 @default.
- W2015624242 cites W2080377664 @default.
- W2015624242 cites W2088547726 @default.
- W2015624242 cites W2093404267 @default.
- W2015624242 cites W2136347799 @default.
- W2015624242 cites W2145031943 @default.
- W2015624242 cites W2212388653 @default.
- W2015624242 cites W2262616897 @default.
- W2015624242 cites W2411407326 @default.
- W2015624242 cites W4240786614 @default.
- W2015624242 doi "https://doi.org/10.1016/0141-0229(92)90174-m" @default.
- W2015624242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1368397" @default.
- W2015624242 hasPublicationYear "1992" @default.
- W2015624242 type Work @default.
- W2015624242 sameAs 2015624242 @default.
- W2015624242 citedByCount "142" @default.
- W2015624242 countsByYear W20156242422012 @default.
- W2015624242 countsByYear W20156242422013 @default.
- W2015624242 countsByYear W20156242422014 @default.
- W2015624242 countsByYear W20156242422015 @default.
- W2015624242 countsByYear W20156242422016 @default.
- W2015624242 countsByYear W20156242422017 @default.
- W2015624242 countsByYear W20156242422018 @default.
- W2015624242 countsByYear W20156242422019 @default.
- W2015624242 countsByYear W20156242422020 @default.
- W2015624242 countsByYear W20156242422021 @default.
- W2015624242 countsByYear W20156242422022 @default.
- W2015624242 crossrefType "journal-article" @default.
- W2015624242 hasAuthorship W2015624242A5030768615 @default.
- W2015624242 hasAuthorship W2015624242A5037902379 @default.
- W2015624242 hasConcept C10138342 @default.
- W2015624242 hasConcept C162324750 @default.
- W2015624242 hasConcept C178790620 @default.
- W2015624242 hasConcept C181199279 @default.
- W2015624242 hasConcept C184133267 @default.
- W2015624242 hasConcept C185592680 @default.
- W2015624242 hasConcept C188027245 @default.
- W2015624242 hasConcept C2775999725 @default.
- W2015624242 hasConcept C2776964284 @default.
- W2015624242 hasConcept C2777516009 @default.
- W2015624242 hasConcept C2779288520 @default.
- W2015624242 hasConcept C2780340462 @default.
- W2015624242 hasConcept C54400483 @default.
- W2015624242 hasConcept C94412978 @default.
- W2015624242 hasConceptScore W2015624242C10138342 @default.
- W2015624242 hasConceptScore W2015624242C162324750 @default.
- W2015624242 hasConceptScore W2015624242C178790620 @default.
- W2015624242 hasConceptScore W2015624242C181199279 @default.
- W2015624242 hasConceptScore W2015624242C184133267 @default.
- W2015624242 hasConceptScore W2015624242C185592680 @default.
- W2015624242 hasConceptScore W2015624242C188027245 @default.
- W2015624242 hasConceptScore W2015624242C2775999725 @default.
- W2015624242 hasConceptScore W2015624242C2776964284 @default.
- W2015624242 hasConceptScore W2015624242C2777516009 @default.
- W2015624242 hasConceptScore W2015624242C2779288520 @default.
- W2015624242 hasConceptScore W2015624242C2780340462 @default.
- W2015624242 hasConceptScore W2015624242C54400483 @default.
- W2015624242 hasConceptScore W2015624242C94412978 @default.
- W2015624242 hasIssue "2" @default.
- W2015624242 hasLocation W20156242421 @default.
- W2015624242 hasLocation W20156242422 @default.
- W2015624242 hasOpenAccess W2015624242 @default.
- W2015624242 hasPrimaryLocation W20156242421 @default.
- W2015624242 hasRelatedWork W1969079757 @default.
- W2015624242 hasRelatedWork W2014630359 @default.
- W2015624242 hasRelatedWork W2014842795 @default.
- W2015624242 hasRelatedWork W2015624242 @default.
- W2015624242 hasRelatedWork W2164474593 @default.
- W2015624242 hasRelatedWork W2357831982 @default.
- W2015624242 hasRelatedWork W2376868106 @default.
- W2015624242 hasRelatedWork W4254439324 @default.
- W2015624242 hasRelatedWork W791154702 @default.
- W2015624242 hasRelatedWork W807352614 @default.
- W2015624242 hasVolume "14" @default.
- W2015624242 isParatext "false" @default.
- W2015624242 isRetracted "false" @default.
- W2015624242 magId "2015624242" @default.
- W2015624242 workType "article" @default.