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- W2015633113 abstract "The two cytochromeb-559 aproproteins of 9.4 kD and 4.5 kD molecular weight have been expressed in vitro using DNA templates containing either the two genes psbE and psbF in tandem or the individual genes. Transcription with E. coli RNA-polymerase or SP6 RNA-polymerase has been followed by translation in E. coli derived lysates. Simultaneous as well as independent synthesis of the apoproteins is possible. A 9.4 kD in vitro translation product has been identified as apoprotein I by immunoprecipitation with a monoclonal antibody specific for the C-terminal part of the 9.4 kD apoprotein of cytochromeb-559. The isolated psbF gene directs the synthesis of a translation product with a molecular weight of 4.5 kD corresponding to apoprotein II. Expression of the psbE gene requires the presence of endogenous regulatory sequences 5′ upstream of psbE, while this is not the case for psbF. Additional in vitro translation products of 5.7 and 2.4 kD molecular weights are synthesized and probably translated from two reading frames starting with two different out-of-phase ATG codons in the nucleotide sequence of the psbE gene." @default.
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- W2015633113 date "1988-01-01" @default.
- W2015633113 modified "2023-09-26" @default.
- W2015633113 title "Characterization and in vitro expression of the cytochromeb-559 genes of barley. II. In vitro transcription and translation" @default.
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- W2015633113 doi "https://doi.org/10.1007/bf02907180" @default.
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