FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015644245> ?p ?o ?g. }

• W2015644245 endingPage "331" @default.
• W2015644245 startingPage "325" @default.
• W2015644245 abstract "Free-standing lipid bilayers are formed in regularly arranged nanopores of 200, 400 and 800 nm in a 300 nm thin hydrophobic silicon nitride membrane separating two fluid compartments. The extraordinary stability of the lipid bilayers allows us to monitor channel formation of the model peptide melittin and alpha-hemolysin from Staphylococcus aureus using electrochemical impedance spectroscopy and chronoamperometry. We observed that melittin channel formation is voltage-dependent and transient, whereas transmembrane heptameric alpha-hemolysin channels in nano-BLMs persist for hours. The onset of alpha-hemolysin-mediated conduction depends on the applied protein concentration and strongly on the diameter of the nanopores. Heptameric channel formation from adsorbed alpha-hemolysin monomers needs more time in bilayers suspended in 200 nm pores compared to bilayers in pores of 400 and 800 nm diameters. Diffusion of sodium ions across alpha-hemolysin channels present in a sufficiently high number in the bilayers was quantitatively and specifically determined using ion selective electrodes. The results demonstrate that relatively small variations of nano-dimensions have a tremendous effect on observable dynamic biomolecular processes. Such nanopore chips are potentially useful as supports for stable lipid bilayers to establish functional assays of membrane proteins needed in basic research and drug discovery." @default.
• W2015644245 created "2016-06-24" @default.
• W2015644245 creator A5028203334 @default.
• W2015644245 creator A5035736293 @default.
• W2015644245 creator A5064857426 @default.
• W2015644245 creator A5067559297 @default.
• W2015644245 date "2009-10-01" @default.
• W2015644245 modified "2023-09-26" @default.
• W2015644245 title "Formation of individual protein channels in lipid bilayers suspended in nanopores" @default.
• W2015644245 cites W1539834786 @default.
• W2015644245 cites W1592730805 @default.
• W2015644245 cites W1594827149 @default.
• W2015644245 cites W1606920356 @default.
• W2015644245 cites W1654340299 @default.
• W2015644245 cites W1965269151 @default.
• W2015644245 cites W1973461864 @default.
• W2015644245 cites W1981452150 @default.
• W2015644245 cites W1983091113 @default.
• W2015644245 cites W1992509240 @default.
• W2015644245 cites W1992737934 @default.
• W2015644245 cites W1996935462 @default.
• W2015644245 cites W2009025716 @default.
• W2015644245 cites W2015784287 @default.
• W2015644245 cites W2018477679 @default.
• W2015644245 cites W2020898688 @default.
• W2015644245 cites W2025117402 @default.
• W2015644245 cites W2030683115 @default.
• W2015644245 cites W2033135248 @default.
• W2015644245 cites W2033795884 @default.
• W2015644245 cites W2038136276 @default.
• W2015644245 cites W2038993805 @default.
• W2015644245 cites W2041901686 @default.
• W2015644245 cites W2044052691 @default.
• W2015644245 cites W2048191546 @default.
• W2015644245 cites W2048340373 @default.
• W2015644245 cites W2051732765 @default.
• W2015644245 cites W2054917853 @default.
• W2015644245 cites W2059205219 @default.
• W2015644245 cites W2059230624 @default.
• W2015644245 cites W2069727454 @default.
• W2015644245 cites W2071628855 @default.
• W2015644245 cites W2074317886 @default.
• W2015644245 cites W2078937546 @default.
• W2015644245 cites W2085381747 @default.
• W2015644245 cites W2085483097 @default.
• W2015644245 cites W2087402763 @default.
• W2015644245 cites W2097212981 @default.
• W2015644245 cites W2103191515 @default.
• W2015644245 cites W2122613015 @default.
• W2015644245 cites W2131909172 @default.
• W2015644245 cites W2886987336 @default.
• W2015644245 doi "https://doi.org/10.1016/j.colsurfb.2009.06.006" @default.
• W2015644245 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19576736" @default.
• W2015644245 hasPublicationYear "2009" @default.
• W2015644245 type Work @default.
• W2015644245 sameAs 2015644245 @default.
• W2015644245 citedByCount "39" @default.
• W2015644245 countsByYear W20156442452012 @default.
• W2015644245 countsByYear W20156442452013 @default.
• W2015644245 countsByYear W20156442452014 @default.
• W2015644245 countsByYear W20156442452015 @default.
• W2015644245 countsByYear W20156442452016 @default.
• W2015644245 countsByYear W20156442452017 @default.
• W2015644245 countsByYear W20156442452018 @default.
• W2015644245 countsByYear W20156442452020 @default.
• W2015644245 countsByYear W20156442452022 @default.
• W2015644245 crossrefType "journal-article" @default.
• W2015644245 hasAuthorship W2015644245A5028203334 @default.
• W2015644245 hasAuthorship W2015644245A5035736293 @default.
• W2015644245 hasAuthorship W2015644245A5064857426 @default.
• W2015644245 hasAuthorship W2015644245A5067559297 @default.
• W2015644245 hasConcept C12554922 @default.
• W2015644245 hasConcept C141795571 @default.
• W2015644245 hasConcept C170493617 @default.
• W2015644245 hasConcept C171250308 @default.
• W2015644245 hasConcept C185592680 @default.
• W2015644245 hasConcept C187632348 @default.
• W2015644245 hasConcept C192562407 @default.
• W2015644245 hasConcept C2778001312 @default.
• W2015644245 hasConcept C37088560 @default.
• W2015644245 hasConcept C39944091 @default.
• W2015644245 hasConcept C41625074 @default.
• W2015644245 hasConcept C50254741 @default.
• W2015644245 hasConcept C55493867 @default.
• W2015644245 hasConcept C86803240 @default.
• W2015644245 hasConceptScore W2015644245C12554922 @default.
• W2015644245 hasConceptScore W2015644245C141795571 @default.
• W2015644245 hasConceptScore W2015644245C170493617 @default.
• W2015644245 hasConceptScore W2015644245C171250308 @default.
• W2015644245 hasConceptScore W2015644245C185592680 @default.
• W2015644245 hasConceptScore W2015644245C187632348 @default.
• W2015644245 hasConceptScore W2015644245C192562407 @default.
• W2015644245 hasConceptScore W2015644245C2778001312 @default.
• W2015644245 hasConceptScore W2015644245C37088560 @default.
• W2015644245 hasConceptScore W2015644245C39944091 @default.
• W2015644245 hasConceptScore W2015644245C41625074 @default.
• W2015644245 hasConceptScore W2015644245C50254741 @default.
• W2015644245 hasConceptScore W2015644245C55493867 @default.

• next