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- W2015654443 abstract "A cDNA encoding mouse thymidylate synthase has been inserted 3′ to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at high frequency. These transformants grew in standard axenic medium without requiring exogenous thymidine. This construct provides a second selectable marker for use in transformation of D. discoideum" @default.
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- W2015654443 title "Complementation of a<i>Dictyoslelium discoideum</i>thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation" @default.
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- W2015654443 doi "https://doi.org/10.1093/nar/17.10.3655" @default.
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