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- W2015672015 abstract "WEAVER, GERALD M., and L. FREDRIC HOUGH. (Rutgers, The State Univ., New Brunswick, N. J.) Seedling growth studies of early-ripening peaches. I. Interrelationship between embryo maturity, growth substances and seedling growth. Amer. Jour. Bot. 46(10): 718-724. Illus. 1959.-Peach fruits of 'Raritan Rose' were harvested 75, 83, 91, 99 and 107 days after full bloom. Embryos were excised, and cultured on artificial media. Growth responses following 8 wk. cold treatment varied considerably with embryo maturity. No correlation existed between seedling survival and the initial responses of shoots. Root growth of 83-day embryos exceeded that of all other stages of maturity. This was reflected in maximum seedling survival and vigor. Initial shoot growth was greatest from embryos harvested at 91 days and at maturity (107 days), but all shoots developed into rosettes which may expand slightly or develop normally from axillary buds. Bioassays of growth substances suggest a strong correlation between early shoot development and auxin concentration. Root growth-responses appeared to be correlated with a promotor-inhibitor balance. The results of wheat coleoptile assays of embryo extracts chromatographed in an isopropanol: ammonia: water solvent (8:1:1) suggest the presence of three growth promotors, in greatest concentration at Rf 0.70, but also at Rf values 0.10 and 0.30. Two growth inhibitors were present in embryo tissue at Rf values 0.27 and 0.96, the former decreasing in concentration with increasing embryo maturity. THE POOR germination of seeds of early-ripening peach varieties is a major obstacle in the development of still earlier-ripening peaches. Low viability was recognized by Connors (1920) as due to incomplete embryo development in early-ripening varieties. Thus, at fruit maturity, the cotyledons had not, as yet, filled the seed coats, and considerable transparent endosperm occupied this space. This situation was quite different from that of later-ripening peaches, in which he described seed maturity as preceding that of the fruits. Tukey (1933) confirmed these findings, reporting non-viability of seeds in varieties which ripened prior to 80 days from pollination. Among those that germinated, there were a large number of abnormal seedlings. The v-iability increased while the percentage of abnormalities decreased in later-ripening varieties. A considerable degree of non-viability was overcome by Davidson (1933, 1934) bv removing the seed coats. by culturing the embryvos on artificial media, and by cold treatment. Modifications in culture techniques by Lesley and Bonner (1952), Kester and Hesse (1955), and Brooks and Hough (1958), have extended the versatilitv of these methods, so that embryos of consistently earlier maturitv have been successfully cultured and germinated. In gen1 Received for publication May 1, 1959. Paper of Journal Series. New Jersey Agricultural Experiment Station, Rutgers, The State University, Department of Horticulture, New Brunswick, New Jersey. The authors wish to express their deep appreciation to Drs. James E. Gunckel, Seymour Shapiro, and Harold Clark for their helpful suggestions in the preparation of the manuscript. 2 Present address: General Cigar Company, Inc., West Hartford, Connecticut. eral, however, embryo culture methods have been disappointing with respect to the development of seedlings for use in the peach breeding program. For, while germination rates are exceedingly high, the seeds fail to develop into normal seedlings. Rather, they are characterized by the development of rosette and curled leaf anomalies (Davidson, 1934; Tukey, 1938). The relationship between embryo maturity and seedling germination and growth was investigated to determine the earliest date for successful culture of embryos of the 'Raritan Rose' peach. A second aspect of this study was to determine the relationship between embryo maturity and naturally occurring growth regulators, on the assumption that the interaction of auxins and inhibitors might account for differences in seedling growth at the various stages of embryo maturity. MATERIALS AND METHODS.-Seeds of 'Raritan Rose,' an early, midseason, white-fleshed peach variety, were harvested at 8-day intervals, beginning 75 days from full bloom and continuing until fruit maturity at 107 days. Seeds at each harvest were divided into 7 replications of 10 seeds each, 3 replications for growth studies and 4 for the analysis of growth substances. All embryos were excised aseptically at harvest and cultured on sterile nutrient agar media containing inorganic salts and 2% sucrose (Brooks and Hough, 1958). Culture tubes were plugged with cotton and sealed with a parafilm cover to prevent contamination and dessication of the media. Following 8-wk. cold treatment at 40 C., the cultures of 3 replications were placed in a greenhouse for germination and seedling growth" @default.
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- W2015672015 date "1959-12-01" @default.
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- W2015672015 title "SEEDLING GROWTH STUDIES OF EARLY-RIPENING PEACHES. I. INTERRELATIONSHIP BETWEEN EMBRYO MATURITY, GROWTH SUBSTANCES AND SEEDLING GROWTH" @default.
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- W2015672015 doi "https://doi.org/10.1002/j.1537-2197.1959.tb07077.x" @default.
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