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• W2015672246 abstract "Gastric enterochromaffin-like (ECL) cells were isolated from rat gastric fundic mucosa by Percoll density-gradient centrifugation and counter-flow elutriation. About 67% of cells in the purified cell suspension were ECL cells, which were reacted with anti-histidine decarboxylase antibody. A23187, a calcium ionophore, at 0.1-10 microM induced histamine release from ECL cell-rich suspension, indicating that the Ca2+ pathway is involved in the mechanism of histamine release from the ECL cells. A23187 at 5 microM significantly increased outward-rectifier cationic current in 62% of cells in the ECL cell-rich factions. A23187-sensitive cells showed acridine orange uptake. In single-channel recordings, a Ca(2+)-dependent outward-rectifier K+ channel of large conductance (146 +/- 22 picosiemens) was found in the cell that showed acridine orange uptake. The channel opened in a voltage-dependent manner at 0.1 microM of intracellular free Ca2+ concentration. These results may suggest that opening of the Ca(2+)-activated K+ channel is one of the steps involved in the mechanism of histamine release in ECL cells." @default.
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• W2015672246 date "1995-10-01" @default.
• W2015672246 modified "2023-09-23" @default.
• W2015672246 title "Ca2+-activated outward-rectifier K+ channels and histamine release by rat gastric enterochromaffin-like cells" @default.
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• W2015672246 doi "https://doi.org/10.1016/0922-4106(95)90137-x" @default.
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