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- W2015683407 abstract "We are developing new analytical devices in order to analyse molecules involved in neuronal cell signalling in individual cells and networks of cells. The unique chip design will allow us to characterize the pattern of synaptic connections between neurons by stimulation with directed microflows to specific individual cells and subsequent detection of cell activity by fluorescence imaging or detection of release (exocytosis) by electrochemical imaging with electrode arrays. We will present the fabrication scheme for this device and preliminary results obtained by culturing pheochromocytoma (PC12) cells onto the chip. The location of the cells will be determined by microcontact printing of surface adhesion proteins (e.g. laminin or poly-L lysine) on the surface of the device. Upon stimulation, intracellular calcium levels in PC12 cells increase and they release dopamine by exocytosis. The former can be monitored with ratiometric fluorescence imaging and the latter can be quantified by electrochemistry and both are non-invasive.These combined measurements represent an important technical development and will help bridge the gap between single cell measurements and those at neuronal systems in organisms. Once characterized, they will be applied to understand patterns of cell signalling and to develop an in vitro model of degenerative diseases like Parkinson's disease." @default.
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- W2015683407 date "2009-02-01" @default.
- W2015683407 modified "2023-09-29" @default.
- W2015683407 title "Multi-detection Device For Studying Neuronal Cell Networks" @default.
- W2015683407 doi "https://doi.org/10.1016/j.bpj.2008.12.2468" @default.
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