Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015683695> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2015683695 endingPage "20" @default.
- W2015683695 startingPage "S15" @default.
- W2015683695 abstract "To develop a competitive polymerase chain reaction technique with which to evaluate the usefulness of HIV-1 level as a marker of response to antiviral treatment.HIV-1 sequences were assessed by competitive polymerase chain reaction in four subjects participating in a double-blind study of monotherapy versus combination therapy with nucleoside analogues.We inserted a mutant construct of the HIV-1 pol sequence into a commercial vector, enabling us to generate known amounts of mutant DNA and RNA for competitive polymerase chain reaction. To measure HIV-1 DNA copies in cells, the mutant DNA fragments were allowed to compete in a 10-fold dilution series with a constant amount of nucleic acid from the subject. To measure HIV-1 RNA copies in plasma, in vitro synthesized mutant RNA was added in a 10-fold dilution series to a constant amount of subject RNA and copy DNA was synthesized. DNA and copy DNA were used as the input for nested pol polymerase chain reaction. Mutant and wild-type amplimers were discriminated by size.The competitive polymerase chain reaction technique has been validated in model experiments and can be used over a broad range (at least 6 logs) of levels. Three of the four subjects showed a decline of 1 log in proviral DNA levels in cells after beginning antiviral treatment. All four showed a decline of at least 1 log in viral RNA levels in plasma, but this decline was transient in one subject.The HIV-1 sequence level is a useful marker in antiviral treatment studies." @default.
- W2015683695 created "2016-06-24" @default.
- W2015683695 creator A5001375239 @default.
- W2015683695 creator A5003406021 @default.
- W2015683695 creator A5006132032 @default.
- W2015683695 creator A5014521750 @default.
- W2015683695 creator A5030081669 @default.
- W2015683695 creator A5048537587 @default.
- W2015683695 creator A5055371178 @default.
- W2015683695 date "1993-11-01" @default.
- W2015683695 modified "2023-09-26" @default.
- W2015683695 title "Use of competitive polymerase chain reaction to determine HIV-1 levels in response to antiviral treatments" @default.
- W2015683695 doi "https://doi.org/10.1097/00002030-199311002-00005" @default.
- W2015683695 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8161441" @default.
- W2015683695 hasPublicationYear "1993" @default.
- W2015683695 type Work @default.
- W2015683695 sameAs 2015683695 @default.
- W2015683695 citedByCount "22" @default.
- W2015683695 crossrefType "journal-article" @default.
- W2015683695 hasAuthorship W2015683695A5001375239 @default.
- W2015683695 hasAuthorship W2015683695A5003406021 @default.
- W2015683695 hasAuthorship W2015683695A5006132032 @default.
- W2015683695 hasAuthorship W2015683695A5014521750 @default.
- W2015683695 hasAuthorship W2015683695A5030081669 @default.
- W2015683695 hasAuthorship W2015683695A5048537587 @default.
- W2015683695 hasAuthorship W2015683695A5055371178 @default.
- W2015683695 hasConcept C104317684 @default.
- W2015683695 hasConcept C143065580 @default.
- W2015683695 hasConcept C153911025 @default.
- W2015683695 hasConcept C156719811 @default.
- W2015683695 hasConcept C159047783 @default.
- W2015683695 hasConcept C24107716 @default.
- W2015683695 hasConcept C48023723 @default.
- W2015683695 hasConcept C49105822 @default.
- W2015683695 hasConcept C54355233 @default.
- W2015683695 hasConcept C552990157 @default.
- W2015683695 hasConcept C67705224 @default.
- W2015683695 hasConcept C82381507 @default.
- W2015683695 hasConcept C86803240 @default.
- W2015683695 hasConceptScore W2015683695C104317684 @default.
- W2015683695 hasConceptScore W2015683695C143065580 @default.
- W2015683695 hasConceptScore W2015683695C153911025 @default.
- W2015683695 hasConceptScore W2015683695C156719811 @default.
- W2015683695 hasConceptScore W2015683695C159047783 @default.
- W2015683695 hasConceptScore W2015683695C24107716 @default.
- W2015683695 hasConceptScore W2015683695C48023723 @default.
- W2015683695 hasConceptScore W2015683695C49105822 @default.
- W2015683695 hasConceptScore W2015683695C54355233 @default.
- W2015683695 hasConceptScore W2015683695C552990157 @default.
- W2015683695 hasConceptScore W2015683695C67705224 @default.
- W2015683695 hasConceptScore W2015683695C82381507 @default.
- W2015683695 hasConceptScore W2015683695C86803240 @default.
- W2015683695 hasLocation W20156836951 @default.
- W2015683695 hasLocation W20156836952 @default.
- W2015683695 hasOpenAccess W2015683695 @default.
- W2015683695 hasPrimaryLocation W20156836951 @default.
- W2015683695 hasRelatedWork W110618481 @default.
- W2015683695 hasRelatedWork W1972497539 @default.
- W2015683695 hasRelatedWork W1998688138 @default.
- W2015683695 hasRelatedWork W2143540931 @default.
- W2015683695 hasRelatedWork W2409845940 @default.
- W2015683695 hasRelatedWork W2414007645 @default.
- W2015683695 hasRelatedWork W2437605097 @default.
- W2015683695 hasRelatedWork W2515311730 @default.
- W2015683695 hasRelatedWork W4250640928 @default.
- W2015683695 hasRelatedWork W4283643599 @default.
- W2015683695 hasVolume "7" @default.
- W2015683695 isParatext "false" @default.
- W2015683695 isRetracted "false" @default.
- W2015683695 magId "2015683695" @default.
- W2015683695 workType "article" @default.