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• W2015686277 abstract "The products of incorporation of uridine-5-3H in embryos from the 8-cell stage to the hatched blastocyst (day 5) were analyzed by sedimentation in sucrose gradients. Ribosomal RNA is a major product of RNA synthesis during this period. Heterodisperse RNA, including a giant-size labile RNA, and 4 S RNA are also synthesized. Pulse labeling and actinomycin chase experiments have led to the identification of a rapidly labeled 39 S RNA species as a precursor of 28 S and 18 S rRNA. Low concentrations of dactinomycin inhibit rRNA synthesis selectively and to an extent proportionate to dose. Uniformly labeled thymidine-14C is incorporated into DNA, RNA, and polysaccharide. These components have been separated and analyzed in CsCl and ethidium bromide-CsCl density gradients. The DNA synthesized is nuclear and labeled to a very high specific activity. The evidence suggests that no extensive replication of mitochondrial DNA takes place during cleavage. A major portion of uniformly labeled thymidine-14C is incorporated into a polysaccharide component during the blastocyst stage (day 4–5). Some properties of this polysaccharide are described." @default.
• W2015686277 created "2016-06-24" @default.
• W2015686277 creator A5042427171 @default.
• W2015686277 date "1970-01-01" @default.
• W2015686277 modified "2023-10-03" @default.
• W2015686277 title "Synthesis of macromolecules in early mouse embryos cultured in vitro: RNA, DNA, and a polysaccharide component" @default.
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• W2015686277 doi "https://doi.org/10.1016/0012-1606(70)90071-0" @default.
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