Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015687682> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2015687682 endingPage "205" @default.
- W2015687682 startingPage "199" @default.
- W2015687682 abstract "High levels of cyclic sugar alcohols (cyclitols) correlate with tolerance to osmotic stress in a number of plant species. A gene encoding a cyclitol biosynthesis enzyme from a halophyte, Mesembryanthemum crystallinum has been introduced into tobacco. The gene, lmt1, encodes a myo-inositol O-methyl transferase that, in M. crystallinum, catalyzes the first step in the stress-induced accumulation of the cyclitol pinitol. Tobacco transformed with the lmt1 cDNA under the control of the CaMV 35S promoter appeared phenotypically normal and exhibited IMT1 enzyme activity. Transformants accumulated a carbohydrate product not detectable in non-transformed control plants. This product was identified by HPLC and NMR as ononitol (1-d-4-O-methyl myo-inositol). Ononitol was a major carbohydrate constituent in leaf tissue of plants expressing the lmt1 gene, accumulating to up to 25% the level of sucrose in transformant seedlings. The identification of ononitol as the IMT1 product and the specific accumulation of this compound in transformed tobacco support a role for ononitol as a stable intermediate in pinitol biosynthesis and indicate that an epimerization activity lacking in tobacco is responsible for the conversion of ononitol to pinitol in M. crystallinum. The production of ononitol in tobacco indicates that plant carbohydrate metabolism is flexible and can accommodate the synthesis and accumulation of non-endogenous metabolites. The transgenic system described here will serve as a useful model to test the ability of cyclitols such as ononitol to confer tolerance to environmental stress in a normally glycophytic plant." @default.
- W2015687682 created "2016-06-24" @default.
- W2015687682 creator A5033708937 @default.
- W2015687682 creator A5040918052 @default.
- W2015687682 creator A5044615389 @default.
- W2015687682 creator A5063869214 @default.
- W2015687682 date "1993-07-01" @default.
- W2015687682 modified "2023-09-23" @default.
- W2015687682 title "Cyclitol production in transgenic tobacco" @default.
- W2015687682 doi "https://doi.org/10.1046/j.1365-313x.1993.04010199.x" @default.
- W2015687682 hasPublicationYear "1993" @default.
- W2015687682 type Work @default.
- W2015687682 sameAs 2015687682 @default.
- W2015687682 citedByCount "83" @default.
- W2015687682 countsByYear W20156876822012 @default.
- W2015687682 countsByYear W20156876822013 @default.
- W2015687682 countsByYear W20156876822014 @default.
- W2015687682 countsByYear W20156876822015 @default.
- W2015687682 countsByYear W20156876822016 @default.
- W2015687682 countsByYear W20156876822017 @default.
- W2015687682 countsByYear W20156876822019 @default.
- W2015687682 countsByYear W20156876822020 @default.
- W2015687682 crossrefType "journal-article" @default.
- W2015687682 hasAuthorship W2015687682A5033708937 @default.
- W2015687682 hasAuthorship W2015687682A5040918052 @default.
- W2015687682 hasAuthorship W2015687682A5044615389 @default.
- W2015687682 hasAuthorship W2015687682A5063869214 @default.
- W2015687682 hasBestOaLocation W20156876821 @default.
- W2015687682 hasConcept C102230213 @default.
- W2015687682 hasConcept C104317684 @default.
- W2015687682 hasConcept C122172344 @default.
- W2015687682 hasConcept C129513315 @default.
- W2015687682 hasConcept C161019410 @default.
- W2015687682 hasConcept C170493617 @default.
- W2015687682 hasConcept C181199279 @default.
- W2015687682 hasConcept C183688256 @default.
- W2015687682 hasConcept C186154404 @default.
- W2015687682 hasConcept C18903297 @default.
- W2015687682 hasConcept C2777427919 @default.
- W2015687682 hasConcept C2778523565 @default.
- W2015687682 hasConcept C2778597767 @default.
- W2015687682 hasConcept C2778977261 @default.
- W2015687682 hasConcept C2780827093 @default.
- W2015687682 hasConcept C553450214 @default.
- W2015687682 hasConcept C55493867 @default.
- W2015687682 hasConcept C7616482 @default.
- W2015687682 hasConcept C86803240 @default.
- W2015687682 hasConceptScore W2015687682C102230213 @default.
- W2015687682 hasConceptScore W2015687682C104317684 @default.
- W2015687682 hasConceptScore W2015687682C122172344 @default.
- W2015687682 hasConceptScore W2015687682C129513315 @default.
- W2015687682 hasConceptScore W2015687682C161019410 @default.
- W2015687682 hasConceptScore W2015687682C170493617 @default.
- W2015687682 hasConceptScore W2015687682C181199279 @default.
- W2015687682 hasConceptScore W2015687682C183688256 @default.
- W2015687682 hasConceptScore W2015687682C186154404 @default.
- W2015687682 hasConceptScore W2015687682C18903297 @default.
- W2015687682 hasConceptScore W2015687682C2777427919 @default.
- W2015687682 hasConceptScore W2015687682C2778523565 @default.
- W2015687682 hasConceptScore W2015687682C2778597767 @default.
- W2015687682 hasConceptScore W2015687682C2778977261 @default.
- W2015687682 hasConceptScore W2015687682C2780827093 @default.
- W2015687682 hasConceptScore W2015687682C553450214 @default.
- W2015687682 hasConceptScore W2015687682C55493867 @default.
- W2015687682 hasConceptScore W2015687682C7616482 @default.
- W2015687682 hasConceptScore W2015687682C86803240 @default.
- W2015687682 hasIssue "1" @default.
- W2015687682 hasLocation W20156876821 @default.
- W2015687682 hasOpenAccess W2015687682 @default.
- W2015687682 hasPrimaryLocation W20156876821 @default.
- W2015687682 hasRelatedWork W1996097380 @default.
- W2015687682 hasRelatedWork W2015687682 @default.
- W2015687682 hasRelatedWork W2048642571 @default.
- W2015687682 hasRelatedWork W2054424210 @default.
- W2015687682 hasRelatedWork W2062338090 @default.
- W2015687682 hasRelatedWork W2108027048 @default.
- W2015687682 hasRelatedWork W2462375631 @default.
- W2015687682 hasRelatedWork W2993479411 @default.
- W2015687682 hasRelatedWork W412475053 @default.
- W2015687682 hasRelatedWork W4360600705 @default.
- W2015687682 hasVolume "4" @default.
- W2015687682 isParatext "false" @default.
- W2015687682 isRetracted "false" @default.
- W2015687682 magId "2015687682" @default.
- W2015687682 workType "article" @default.