Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015701579> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W2015701579 abstract "Biochemical studies on the degradation of D-galactonate by cell-free extracts of Aspergillus niger indicated that the pH value and temperature optima were 8.0 and 7.5 and 40 degrees C and 50 degrees C for the two enzymes responsible for this degradation namely D-galactonate dehydratase and 2-keto-3-deoxy-D-galactonate (KDGal) aldolase respectively. The effects of the nature of the buffer substance, buffer molarity and enzyme concentration were also studied. Thermal stability behaviour studies show that D-galactonate dehydratase was stable at 40 degrees C for 60 minutes and about 41%, 80% and 90% of enzyme activity were lost by exposing the extracts to 50 degrees C, 60 degrees C and 70 degrees C, respectively, for the same period. However, exposing the extracts to 70 degrees C after 60 minutes caused a complete inhibition for KDGal aldolase activity and a gradual decrease in activity was noticed by incubation the extracts at 60 degrees C. The results of freezing and thawing treatment indicated that KDGal aldolase was more stable than D-galactonate dehydratase in this respect, as only 27% of enzyme activity was lost after 5 days of storage at -5 degrees C. Dialyzing the extracts significantly affects KDGal formation from D-galactonate. Results obtained also indicated the non-requirement of metal ions for activation of KDGal aldolase. On the other, hand D-galactonate dehydratase has a requirement for Mg++ and Mn++, however ZnSO4 and HgCl2 caused a complete inhibition of the enzymatic activity of this enzyme." @default.
- W2015701579 created "2016-06-24" @default.
- W2015701579 creator A5027229960 @default.
- W2015701579 creator A5069193310 @default.
- W2015701579 date "2001-07-01" @default.
- W2015701579 modified "2023-09-23" @default.
- W2015701579 title "Factors affecting D-galactonate degradation by extracts of Aspergillus niger" @default.
- W2015701579 doi "https://doi.org/10.1002/1521-4028(200107)41:3/4<149::aid-jobm149>3.0.co;2-t" @default.
- W2015701579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11512447" @default.
- W2015701579 hasPublicationYear "2001" @default.
- W2015701579 type Work @default.
- W2015701579 sameAs 2015701579 @default.
- W2015701579 citedByCount "3" @default.
- W2015701579 crossrefType "journal-article" @default.
- W2015701579 hasAuthorship W2015701579A5027229960 @default.
- W2015701579 hasAuthorship W2015701579A5069193310 @default.
- W2015701579 hasConcept C181199279 @default.
- W2015701579 hasConcept C185592680 @default.
- W2015701579 hasConcept C2775960407 @default.
- W2015701579 hasConcept C2781374117 @default.
- W2015701579 hasConcept C28165981 @default.
- W2015701579 hasConcept C28212737 @default.
- W2015701579 hasConcept C43617362 @default.
- W2015701579 hasConcept C55493867 @default.
- W2015701579 hasConceptScore W2015701579C181199279 @default.
- W2015701579 hasConceptScore W2015701579C185592680 @default.
- W2015701579 hasConceptScore W2015701579C2775960407 @default.
- W2015701579 hasConceptScore W2015701579C2781374117 @default.
- W2015701579 hasConceptScore W2015701579C28165981 @default.
- W2015701579 hasConceptScore W2015701579C28212737 @default.
- W2015701579 hasConceptScore W2015701579C43617362 @default.
- W2015701579 hasConceptScore W2015701579C55493867 @default.
- W2015701579 hasLocation W20157015791 @default.
- W2015701579 hasLocation W20157015792 @default.
- W2015701579 hasOpenAccess W2015701579 @default.
- W2015701579 hasPrimaryLocation W20157015791 @default.
- W2015701579 hasRelatedWork W1606347250 @default.
- W2015701579 hasRelatedWork W1962968308 @default.
- W2015701579 hasRelatedWork W1965932661 @default.
- W2015701579 hasRelatedWork W1966622660 @default.
- W2015701579 hasRelatedWork W1978746292 @default.
- W2015701579 hasRelatedWork W1987671987 @default.
- W2015701579 hasRelatedWork W2019427158 @default.
- W2015701579 hasRelatedWork W2024084298 @default.
- W2015701579 hasRelatedWork W2042445951 @default.
- W2015701579 hasRelatedWork W2071077805 @default.
- W2015701579 hasRelatedWork W2078042749 @default.
- W2015701579 hasRelatedWork W2325540509 @default.
- W2015701579 hasRelatedWork W2335897426 @default.
- W2015701579 hasRelatedWork W2338276728 @default.
- W2015701579 hasRelatedWork W2339682051 @default.
- W2015701579 hasRelatedWork W2374704023 @default.
- W2015701579 hasRelatedWork W2397496872 @default.
- W2015701579 hasRelatedWork W2558037834 @default.
- W2015701579 hasRelatedWork W2950300282 @default.
- W2015701579 hasRelatedWork W2513285915 @default.
- W2015701579 isParatext "false" @default.
- W2015701579 isRetracted "false" @default.
- W2015701579 magId "2015701579" @default.
- W2015701579 workType "article" @default.