Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015779168> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2015779168 endingPage "O111.011031" @default.
- W2015779168 startingPage "O111.011031" @default.
- W2015779168 abstract "Western blotting is a powerful and widely used method, but limitations in detection sensitivity and specificity, and dependence upon high quality antibodies to detect targeted proteins, are hurdles to overcome. The in situ proximity ligation assay, based on dual antibody recognition and powerful localized signal amplification, offers increased detection sensitivity and specificity, along with an ability to identify complex targets such as phosphorylated or interacting proteins. Here we have applied the in situ proximity ligation assay mechanism in Western blotting. This combination allowed the use of isothermal rolling circle amplification of DNA molecules formed in target-specific ligation reaction, for 16-fold or greater increase in detection sensitivity. The increased specificity because of dual antibody recognition ensured highly selective assays, detecting the specific band when combinations of two cross-reactive antitubulin antibodies were used (i.e. both producing distinct nonspecific bands in traditional Western blotting). We also demonstrated detection of phosphorylated platelet-derived growth factor receptor β by proximity ligation with one antibody directed against the receptor and another directed against the phosphorylated tyrosine residue. This avoided the need for stripping and re-probing the membrane or aligning two separate traditional blots. We demonstrate that the high-performance in situ proximity ligation-based Western blotting described herein is compatible with detection via enhanced chemiluminescence and fluorescence detection systems, and can thus be readily employed in any laboratory." @default.
- W2015779168 created "2016-06-24" @default.
- W2015779168 creator A5022012433 @default.
- W2015779168 creator A5039741791 @default.
- W2015779168 creator A5045543302 @default.
- W2015779168 creator A5049251645 @default.
- W2015779168 creator A5049540208 @default.
- W2015779168 creator A5058831434 @default.
- W2015779168 creator A5068073663 @default.
- W2015779168 creator A5068488665 @default.
- W2015779168 creator A5072680425 @default.
- W2015779168 creator A5085565436 @default.
- W2015779168 date "2011-11-01" @default.
- W2015779168 modified "2023-10-14" @default.
- W2015779168 title "Western Blotting via Proximity Ligation for High Performance Protein Analysis" @default.
- W2015779168 cites W1967204260 @default.
- W2015779168 cites W1992496728 @default.
- W2015779168 cites W2008832201 @default.
- W2015779168 cites W2033692444 @default.
- W2015779168 cites W2039110116 @default.
- W2015779168 cites W2082107683 @default.
- W2015779168 cites W2092956762 @default.
- W2015779168 cites W2095176848 @default.
- W2015779168 cites W2105205198 @default.
- W2015779168 cites W2111817213 @default.
- W2015779168 cites W2137166024 @default.
- W2015779168 cites W2171893964 @default.
- W2015779168 cites W4234067205 @default.
- W2015779168 doi "https://doi.org/10.1074/mcp.o111.011031" @default.
- W2015779168 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3226413" @default.
- W2015779168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21813417" @default.
- W2015779168 hasPublicationYear "2011" @default.
- W2015779168 type Work @default.
- W2015779168 sameAs 2015779168 @default.
- W2015779168 citedByCount "22" @default.
- W2015779168 countsByYear W20157791682012 @default.
- W2015779168 countsByYear W20157791682013 @default.
- W2015779168 countsByYear W20157791682014 @default.
- W2015779168 countsByYear W20157791682015 @default.
- W2015779168 countsByYear W20157791682016 @default.
- W2015779168 countsByYear W20157791682017 @default.
- W2015779168 countsByYear W20157791682018 @default.
- W2015779168 countsByYear W20157791682021 @default.
- W2015779168 countsByYear W20157791682022 @default.
- W2015779168 countsByYear W20157791682023 @default.
- W2015779168 crossrefType "journal-article" @default.
- W2015779168 hasAuthorship W2015779168A5022012433 @default.
- W2015779168 hasAuthorship W2015779168A5039741791 @default.
- W2015779168 hasAuthorship W2015779168A5045543302 @default.
- W2015779168 hasAuthorship W2015779168A5049251645 @default.
- W2015779168 hasAuthorship W2015779168A5049540208 @default.
- W2015779168 hasAuthorship W2015779168A5058831434 @default.
- W2015779168 hasAuthorship W2015779168A5068073663 @default.
- W2015779168 hasAuthorship W2015779168A5068488665 @default.
- W2015779168 hasAuthorship W2015779168A5072680425 @default.
- W2015779168 hasAuthorship W2015779168A5085565436 @default.
- W2015779168 hasBestOaLocation W20157791681 @default.
- W2015779168 hasConcept C104317684 @default.
- W2015779168 hasConcept C153911025 @default.
- W2015779168 hasConcept C170493617 @default.
- W2015779168 hasConcept C179437574 @default.
- W2015779168 hasConcept C185592680 @default.
- W2015779168 hasConcept C2776243700 @default.
- W2015779168 hasConcept C55493867 @default.
- W2015779168 hasConcept C85265743 @default.
- W2015779168 hasConcept C86803240 @default.
- W2015779168 hasConceptScore W2015779168C104317684 @default.
- W2015779168 hasConceptScore W2015779168C153911025 @default.
- W2015779168 hasConceptScore W2015779168C170493617 @default.
- W2015779168 hasConceptScore W2015779168C179437574 @default.
- W2015779168 hasConceptScore W2015779168C185592680 @default.
- W2015779168 hasConceptScore W2015779168C2776243700 @default.
- W2015779168 hasConceptScore W2015779168C55493867 @default.
- W2015779168 hasConceptScore W2015779168C85265743 @default.
- W2015779168 hasConceptScore W2015779168C86803240 @default.
- W2015779168 hasIssue "11" @default.
- W2015779168 hasLocation W20157791681 @default.
- W2015779168 hasLocation W20157791682 @default.
- W2015779168 hasLocation W20157791683 @default.
- W2015779168 hasLocation W20157791684 @default.
- W2015779168 hasOpenAccess W2015779168 @default.
- W2015779168 hasPrimaryLocation W20157791681 @default.
- W2015779168 hasRelatedWork W1972908366 @default.
- W2015779168 hasRelatedWork W1999757248 @default.
- W2015779168 hasRelatedWork W2056436268 @default.
- W2015779168 hasRelatedWork W2329682587 @default.
- W2015779168 hasRelatedWork W2375462734 @default.
- W2015779168 hasRelatedWork W2490828730 @default.
- W2015779168 hasRelatedWork W2524960371 @default.
- W2015779168 hasRelatedWork W2753251897 @default.
- W2015779168 hasRelatedWork W3140136072 @default.
- W2015779168 hasRelatedWork W3155426618 @default.
- W2015779168 hasVolume "10" @default.
- W2015779168 isParatext "false" @default.
- W2015779168 isRetracted "false" @default.
- W2015779168 magId "2015779168" @default.
- W2015779168 workType "article" @default.