FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015796743> ?p ?o ?g. }

• W2015796743 endingPage "103" @default.
• W2015796743 startingPage "102" @default.
• W2015796743 abstract "This review article discusses several technologies using the power of genetic analysis that were used to rapidly detect microbial contamination, provide a more accurate identification of microorganisms, understand the microbial diversity in cleanroom environments, and optimize pharmaceutical processes. Nucleic acids were extracted and purified from enrichment cultures of product suspensions, growing cultures, microbial isolates, and directly from water and cleanroom surfaces. The extracted microbial DNA was either amplified using different polymerase chain reaction (PCR) assays or analyzed using DNA microarray technology. PCR assays were targeting genes coding for structural proteins, ribosomal subunits, or catabolic enzymes. Different PCR formats were tested ranging from single gene amplification to multiplexing. The detection of the amplified fragments was performed by qualitative or quantitative analysis. In some studies the amplified products were cloned/sequenced or sequenced to accurately determine the identity and diversity of the microbes present in the samples. DNA microarray analyses provided a higher multiplexing capability than PCR assays, as more than 3000 genes were analyzed in a single reaction, providing a higher resolution and specificity of the microorganisms present in cleanroom environments and pharmaceutical samples. <i>LAY ABSTRACT:</i> Molecular technologies using genetic sequences were used to rapidly detect microbial contamination, provide a more accurate identification of microorganisms, understand the types of microbes in pharmaceutical laboratories, and make pharmaceutical processes more efficient. The microbial genetic sequences were extracted from different types of samples. Then the extracted sequences were analyzed by looking for specific signatures to determine the identity and function of the different microbes in products, environments, and processes. Genetic analyses provided a better description of the different types of microbes present in pharmaceutical samples with a rapid quality evaluation of products and processes." @default.
• W2015796743 created "2016-06-24" @default.
• W2015796743 creator A5032091326 @default.
• W2015796743 date "1981-02-01" @default.
• W2015796743 modified "2023-09-26" @default.
• W2015796743 title "Discriminatory indices in rheumatoid arthritis." @default.
• W2015796743 cites W1964213995 @default.
• W2015796743 cites W1974489459 @default.
• W2015796743 cites W2010588224 @default.
• W2015796743 cites W2018399656 @default.
• W2015796743 cites W2020502392 @default.
• W2015796743 cites W2045282875 @default.
• W2015796743 cites W2045489072 @default.
• W2015796743 cites W2053704326 @default.
• W2015796743 cites W2090788985 @default.
• W2015796743 cites W2099008143 @default.
• W2015796743 cites W59039644 @default.
• W2015796743 doi "https://doi.org/10.1136/ard.40.1.102" @default.
• W2015796743 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1000669" @default.
• W2015796743 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7469520" @default.
• W2015796743 hasPublicationYear "1981" @default.
• W2015796743 type Work @default.
• W2015796743 sameAs 2015796743 @default.
• W2015796743 citedByCount "0" @default.
• W2015796743 crossrefType "journal-article" @default.
• W2015796743 hasAuthorship W2015796743A5032091326 @default.
• W2015796743 hasBestOaLocation W20157967431 @default.
• W2015796743 hasConcept C104317684 @default.
• W2015796743 hasConcept C121332964 @default.
• W2015796743 hasConcept C127094628 @default.
• W2015796743 hasConcept C150194340 @default.
• W2015796743 hasConcept C175605896 @default.
• W2015796743 hasConcept C49105822 @default.
• W2015796743 hasConcept C523546767 @default.
• W2015796743 hasConcept C54355233 @default.
• W2015796743 hasConcept C62520636 @default.
• W2015796743 hasConcept C70721500 @default.
• W2015796743 hasConcept C86803240 @default.
• W2015796743 hasConcept C89423630 @default.
• W2015796743 hasConcept C95371953 @default.
• W2015796743 hasConceptScore W2015796743C104317684 @default.
• W2015796743 hasConceptScore W2015796743C121332964 @default.
• W2015796743 hasConceptScore W2015796743C127094628 @default.
• W2015796743 hasConceptScore W2015796743C150194340 @default.
• W2015796743 hasConceptScore W2015796743C175605896 @default.
• W2015796743 hasConceptScore W2015796743C49105822 @default.
• W2015796743 hasConceptScore W2015796743C523546767 @default.
• W2015796743 hasConceptScore W2015796743C54355233 @default.
• W2015796743 hasConceptScore W2015796743C62520636 @default.
• W2015796743 hasConceptScore W2015796743C70721500 @default.
• W2015796743 hasConceptScore W2015796743C86803240 @default.
• W2015796743 hasConceptScore W2015796743C89423630 @default.
• W2015796743 hasConceptScore W2015796743C95371953 @default.
• W2015796743 hasIssue "1" @default.
• W2015796743 hasLocation W20157967431 @default.
• W2015796743 hasLocation W20157967432 @default.
• W2015796743 hasLocation W20157967433 @default.
• W2015796743 hasLocation W20157967434 @default.
• W2015796743 hasOpenAccess W2015796743 @default.
• W2015796743 hasPrimaryLocation W20157967431 @default.
• W2015796743 hasRelatedWork W1905932766 @default.
• W2015796743 hasRelatedWork W1970453278 @default.
• W2015796743 hasRelatedWork W2000931842 @default.
• W2015796743 hasRelatedWork W2001677368 @default.
• W2015796743 hasRelatedWork W2002128513 @default.
• W2015796743 hasRelatedWork W2016446411 @default.
• W2015796743 hasRelatedWork W2076099888 @default.
• W2015796743 hasRelatedWork W2080565722 @default.
• W2015796743 hasRelatedWork W2097265502 @default.
• W2015796743 hasRelatedWork W2111172999 @default.
• W2015796743 hasVolume "40" @default.
• W2015796743 isParatext "false" @default.
• W2015796743 isRetracted "false" @default.
• W2015796743 magId "2015796743" @default.
• W2015796743 workType "article" @default.