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- W2015808209 abstract "Abstract Rat ventral prostate is very rich in polyamines that, during homogenization, cause aggregation of subcellular organelles jeopardizing subfractionation studies of this organ. 1. 1) Heparin added in vitro to rat ventral prostate subcellular fractions to sequester polyamines exerts the following effects: a) minimizes aggregation of most membranous material and lowers its sedimentation rate; b) removes some ribosomes from the microsomal vesicles; c) causes disruption of nuclei and an apparent subnuclear fractionation. 2. 2) After removal of nucleic which interfere in fractionation, heparin was added to subfractionate the other cellular components. Heparin concentration and centrifugation conditions were tested to set up a reliable method of subcellular fractionation. 3. 3) The distribution of the marker enzymes galactosyltransferase (Golgi apparatus) and acid phosphatase (lysosomes and secretory granules) were checked to assess the usefulness of the method." @default.
- W2015808209 created "2016-06-24" @default.
- W2015808209 creator A5024510103 @default.
- W2015808209 date "1982-04-01" @default.
- W2015808209 modified "2023-09-27" @default.
- W2015808209 title "Effect of heparin on the subcellular fractionation of a polyamines rich organ (rat ventral prostate)" @default.
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- W2015808209 doi "https://doi.org/10.1016/0006-291x(82)90924-x" @default.
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