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- W2015847100 abstract "Enhanced Haemolysis Agar (EHA) medium has been developed for the selective isolation ofListeria monocytogenes from food enrichments. It is based on the selective agents of the Oxford medium (Curtis et al. 1989a), with the addition of sheep blood (5%), 4-methylumbelliferyl-β-d-glucoside (50 mg l−1) and sphingomyelinase (10 units/l−1). The medium contains no esculin or ferric citrate and the lithium chloride concentration is reduced to 1%.L. monocytogenes colonies are distinguished by their light blue fluorescence and yellow centre (in UV 366nm), colony morphology and the presence of a distinct zone of haemolysis around the colony, indicating the production of the CAMP factor. Listeria innocua does not produce haemolysis on this medium and therefore cannot be confused withL. monocytogenes. Haemolysis positiveListeria seeligeri, although found relatively rarely in foods, can be distinguished by performing a xylose fermentation test after isolation on EHA. Strains ofListeria ivanovii from our collection failed to grow on this medium. Although haemolysis-negative and CAMP-negativeL. monocytogenes may not be detected by this medium, their apparent lack of pathogenicity makes them of limited interest to food microbiologists." @default.
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- W2015847100 date "1991-03-01" @default.
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- W2015847100 title "Enhanced haemolysis agar (EHA)—an improved selective and differential medium for isolation ofListeria monocytogenes" @default.
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- W2015847100 doi "https://doi.org/10.1016/0740-0020(91)90015-t" @default.
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