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- W2015867939 abstract "Using mechanical cell properties measured by micromanipulation, and a model of cell distortion in laminar flow fields, a method has been developed for predicting disruption of animal cells by laminar shear stresses. Predictions of the model were compared with measured losses of cell number and viability of TB/C3 murine hybridomas sheared in a cone and plate viscometer at shear rates up to 3950 s(-1), and shear stresses up to 600 Nm(-2), achieved by enhancement of viscosity with dextran. In all cases, the experimental, results and predictions were within 30%. Such excellent agreement suggests it might be possible to use micromanipulation measurements of animal cell mechanical properties to predict cell damage in more complex flow fields, such as those in bioreactors." @default.
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- W2015867939 date "1992-11-01" @default.
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- W2015867939 title "Estimation of disruption of animal cells by laminar shear stress" @default.
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- W2015867939 doi "https://doi.org/10.1002/bit.260400903" @default.
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