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- W2015877438 abstract "Experimental conditions for extraction of extracellular catalase from soil were studied by using tetra-sodium pyrophosphate as extractive solution. Mixture of soil with 0.01 M pyrophosphate in a 1:2 ratio (w/v), adjustment of pH to 7.0–7.3, 18 h of reciprocating agitation at 1.05 × g (60 rpm) and bacteriological filtration after centrifugation were the optimum conditions for extraction of soil fractions with catalase activity. Concentration and pH of pyrophosphate were the factors with the most influence on the extraction yields. Some aspects of pH variation in successive extractions of enzyme and differences between the use of extractive solutions at a same buffer pH or control of extraction pH are reported." @default.
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- W2015877438 date "1988-03-01" @default.
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- W2015877438 title "Extraction of Catalase From Soil" @default.
- W2015877438 doi "https://doi.org/10.2136/sssaj1988.03615995005200020018x" @default.
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