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• W2015888582 abstract "It is commonly accepted that the DNA of Drosophila melanogaster does not contain 5-methylcytosine, which is essential in the development of most eukaryotes. We have developed a new, highly specific and sensitive assay to detect the presence of 5-methylcytosine in genomic DNA. The DNA is degraded to nucleosides, 5-methylcytosine purified by HPLC and, for detection by 1D- and 2D-TLC, radiolabeled using deoxynucleoside kinase and [gamma-(32)P]ATP. Using this assay, we show here that 5-methylcytosine occurs in the DNA of D. melanogaster at a level of approximately 1 in 1000-2000 cytosine residues in adult flies. DNA methylation is detectable in all stages of D.melanogaster development." @default.
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• W2015888582 date "2000-12-15" @default.
• W2015888582 modified "2023-10-16" @default.
• W2015888582 title "DNA of Drosophila melanogaster contains 5-methylcytosine" @default.
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• W2015888582 doi "https://doi.org/10.1093/emboj/19.24.6918" @default.
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