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- W2015952004 abstract "Myosin's subfragment 1 and three light chains were chromatographed rapidly at room temperature on Spherogel TSK-type columns, recently developed for steric-exclusion high-performance liquid chromatography, with no adsorption of the proteins to the columns or loss of enzymic activity. Crude preparations of subfragment 1 were extensively purified on Spherogel TSK SW-3000 columns. Unresolved α-chymotryptic-subfragment 1 was separated into its two isoenzymes despite only a small difference in molecular weights, presumably because of conformational differences. Light chain 2 was purified from a mixture of all three light chains by chromatography on a Spherogel TSK SW-2000 column; light chains 1 and 3 eluted as two distinct complexes having ratios of 2:1 and 1:1." @default.
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- W2015952004 date "1980-09-01" @default.
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- W2015952004 title "High-performance liquid chromatography of myosin's subfragment 1 and light chains on Spherogel TSK-type columns" @default.
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- W2015952004 doi "https://doi.org/10.1016/s0021-9673(00)81237-6" @default.
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