FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015963367> ?p ?o ?g. }

• W2015963367 endingPage "124" @default.
• W2015963367 startingPage "118" @default.
• W2015963367 abstract "ADv cells are Chinese hamster ovary (CHO) cell variants which cannot adhere to fibronectin coated substrata (Harper amp; Juliano: J. Cell Biol., 1980; Nature 1981a, b). We have shown that the defect in some clones of ADv cells is distal to the initial interaction between fibronectin and its cell surface receptors (Cheung and Juliano: Exp. Cell Res., 1984), and that it extends to fibronectin mediated aggregation and endocytosis. The adhesion defect in some ADv clones can be corrected by raising intracellular cAMP levels (Cheung & Juliano: J. Cell Physiol., 1985). Here we examine the protein kinase activities and phosphorylation patterns in an adhesion defective variant clone ADv F11CA11. Analysis of the cAMP dependent protein kinase activity (cAdPK) in crude extracts of F11CA11 cells shows an apparent increase in K (activation) as compared to wild type (WT) CHO cell extracts. Further, the DE-52 cellulose chromatography profile of cAdPK in the F11CA11 variant is markedly different from WT in that the type I cAdPK peak elicited by 1 μM cAMP is essentially missing in F11CA11, while the type II cAdPK peak is similar to that in WT. Raising the cAMP level to 100 μM elicits a type I peak in F11CA11 with about 45% of the activity of the WT peak. Binding studies with 3H-cAMP reveal that the type I peak in F11CA11 has a Kd of 1.7 times; 10−8 M as compared to 2.0 × 10−9 M for WT, whereas the type II peak Kd is approximately 1 × 10−9 M for both WT and F11CA11. Two-dimensional polyacrylamide gel analysis of 32Pi labeled WT cells and F11CA11 cells with or without cAMP treatment reveals the presence of a protein(s) of 50 kilodaltons which is phosphorylated in WT cells and in cAMP treated F11CA11 cells but not in untreated F11CA11 cells. These findings, coupled with our previous observations, strongly indicate taht the adhesion defect in ADvF11CA11 cells is associated with an altered type I cAdPK having lower affinity for cAMP. At normal cellular cAMP levels this enzyme fails to phosphorylate one or more critical protein substrates; however, by raising internal cAMP levels, the defect can be overcome. Thus type I cAdPK seems to play an important role in the regulation of fibronectin mediated cell adhesion, cell aggregation, and endocytosis." @default.
• W2015963367 created "2016-06-24" @default.
• W2015963367 creator A5031351064 @default.
• W2015963367 creator A5031723779 @default.
• W2015963367 creator A5058344546 @default.
• W2015963367 date "1987-01-01" @default.
• W2015963367 modified "2023-10-16" @default.
• W2015963367 title "Altered type I protein kinase in adhesion defective CHO cell variants" @default.
• W2015963367 cites W1547291340 @default.
• W2015963367 cites W1583571376 @default.
• W2015963367 cites W177575448 @default.
• W2015963367 cites W1806064940 @default.
• W2015963367 cites W1975685135 @default.
• W2015963367 cites W1985127862 @default.
• W2015963367 cites W1988041354 @default.
• W2015963367 cites W1996466550 @default.
• W2015963367 cites W2026373274 @default.
• W2015963367 cites W2028824492 @default.
• W2015963367 cites W2030349770 @default.
• W2015963367 cites W2047055721 @default.
• W2015963367 cites W2062145172 @default.
• W2015963367 cites W2065051915 @default.
• W2015963367 cites W2069161066 @default.
• W2015963367 cites W2076065807 @default.
• W2015963367 cites W2086155704 @default.
• W2015963367 cites W2090080663 @default.
• W2015963367 cites W2100837269 @default.
• W2015963367 cites W2108175672 @default.
• W2015963367 cites W2123426218 @default.
• W2015963367 cites W2179433498 @default.
• W2015963367 cites W949119160 @default.
• W2015963367 cites W1544190520 @default.
• W2015963367 doi "https://doi.org/10.1002/jcp.1041300117" @default.
• W2015963367 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3027105" @default.
• W2015963367 hasPublicationYear "1987" @default.
• W2015963367 type Work @default.
• W2015963367 sameAs 2015963367 @default.
• W2015963367 citedByCount "12" @default.
• W2015963367 crossrefType "journal-article" @default.
• W2015963367 hasAuthorship W2015963367A5031351064 @default.
• W2015963367 hasAuthorship W2015963367A5031723779 @default.
• W2015963367 hasAuthorship W2015963367A5058344546 @default.
• W2015963367 hasConcept C104317684 @default.
• W2015963367 hasConcept C1491633281 @default.
• W2015963367 hasConcept C153911025 @default.
• W2015963367 hasConcept C170493617 @default.
• W2015963367 hasConcept C175656101 @default.
• W2015963367 hasConcept C184235292 @default.
• W2015963367 hasConcept C185592680 @default.
• W2015963367 hasConcept C189014844 @default.
• W2015963367 hasConcept C28005876 @default.
• W2015963367 hasConcept C55493867 @default.
• W2015963367 hasConcept C79879829 @default.
• W2015963367 hasConcept C81089528 @default.
• W2015963367 hasConcept C85789140 @default.
• W2015963367 hasConcept C86492073 @default.
• W2015963367 hasConcept C86803240 @default.
• W2015963367 hasConcept C95444343 @default.
• W2015963367 hasConcept C97029542 @default.
• W2015963367 hasConceptScore W2015963367C104317684 @default.
• W2015963367 hasConceptScore W2015963367C1491633281 @default.
• W2015963367 hasConceptScore W2015963367C153911025 @default.
• W2015963367 hasConceptScore W2015963367C170493617 @default.
• W2015963367 hasConceptScore W2015963367C175656101 @default.
• W2015963367 hasConceptScore W2015963367C184235292 @default.
• W2015963367 hasConceptScore W2015963367C185592680 @default.
• W2015963367 hasConceptScore W2015963367C189014844 @default.
• W2015963367 hasConceptScore W2015963367C28005876 @default.
• W2015963367 hasConceptScore W2015963367C55493867 @default.
• W2015963367 hasConceptScore W2015963367C79879829 @default.
• W2015963367 hasConceptScore W2015963367C81089528 @default.
• W2015963367 hasConceptScore W2015963367C85789140 @default.
• W2015963367 hasConceptScore W2015963367C86492073 @default.
• W2015963367 hasConceptScore W2015963367C86803240 @default.
• W2015963367 hasConceptScore W2015963367C95444343 @default.
• W2015963367 hasConceptScore W2015963367C97029542 @default.
• W2015963367 hasIssue "1" @default.
• W2015963367 hasLocation W20159633671 @default.
• W2015963367 hasLocation W20159633672 @default.
• W2015963367 hasOpenAccess W2015963367 @default.
• W2015963367 hasPrimaryLocation W20159633671 @default.
• W2015963367 hasRelatedWork W1967168691 @default.
• W2015963367 hasRelatedWork W1985822908 @default.
• W2015963367 hasRelatedWork W2017530429 @default.
• W2015963367 hasRelatedWork W2020199193 @default.
• W2015963367 hasRelatedWork W2040010734 @default.
• W2015963367 hasRelatedWork W2059128760 @default.
• W2015963367 hasRelatedWork W2076670732 @default.
• W2015963367 hasRelatedWork W3021061361 @default.
• W2015963367 hasRelatedWork W4233800562 @default.
• W2015963367 hasRelatedWork W4255052972 @default.
• W2015963367 hasVolume "130" @default.
• W2015963367 isParatext "false" @default.
• W2015963367 isRetracted "false" @default.
• W2015963367 magId "2015963367" @default.
• W2015963367 workType "article" @default.