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• W2015969705 abstract "Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2'-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs." @default.
• W2015969705 created "2016-06-24" @default.
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• W2015969705 date "2005-02-11" @default.
• W2015969705 modified "2023-10-18" @default.
• W2015969705 title "Methylation as a Crucial Step in Plant microRNA Biogenesis" @default.
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• W2015969705 doi "https://doi.org/10.1126/science.1107130" @default.
• W2015969705 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5137370" @default.
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