FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015972426> ?p ?o ?g. }

• W2015972426 endingPage "280" @default.
• W2015972426 startingPage "267" @default.
• W2015972426 abstract "Mutants of minute virus of mice (MVM) which express truncated forms of the NS2 polypeptide are known to exhibit a host range defect, replicating productively in transformed human cells but not in cells from their normal murine host. To explore this deficiency we generated viruses with translation termination codons at various positions in the second exon of NS2. In human cells these mutants were viable, but showed a late defect in progeny virion release which put them at a selective disadvantage compared to the wildtype. In murine cells, however, duplex viral DNA amplification was reduced to 5% of wildtype levels and single-strand DNA synthesis was undetectable. These deficiencies could not be attributed to a failure to initiate infection or to a generalized defect in viral gene expression, since the viral replicator protein NS1 was expressed to normal or elevated levels early in infection. In contrast, truncated NS2 gene products failed to accumulate, so that each mutant exhibited a similar NS2-null phenotype. Expression of the capsid polypeptides VP1 and VP2 and their subsequent assembly into intact particles were examined in detail. Synchronized infected cell populations labeled under pulse–chase conditions were analyzed by differential immunoprecipitation of native or denatured extracts using antibodies which discriminated between intact particles and isolated polypeptide chains. These analyses showed that at early times in infection, capsid protein synthesis and stability were normal, but particle assembly was impaired. Unassembled VP proteins were retained in the cell for several hours, but as the unprocessed material accumulated, capsid protein synthesis progressively diminished, so that at later times relatively few VP molecules were synthesized. Thus in NS2-null infections of mouse cells there is a major primary defect in the folding or assembly processes required for effective capsid production." @default.
• W2015972426 created "2016-06-24" @default.
• W2015972426 creator A5005437319 @default.
• W2015972426 creator A5019410432 @default.
• W2015972426 creator A5033180835 @default.
• W2015972426 creator A5061059543 @default.
• W2015972426 creator A5067304973 @default.
• W2015972426 date "1997-05-01" @default.
• W2015972426 modified "2023-10-17" @default.
• W2015972426 title "The NS2 Polypeptide of Parvovirus MVM Is Required for Capsid Assembly in Murine Cells" @default.
• W2015972426 cites W1415981329 @default.
• W2015972426 cites W1480402597 @default.
• W2015972426 cites W1484473517 @default.
• W2015972426 cites W1529305757 @default.
• W2015972426 cites W1548822658 @default.
• W2015972426 cites W1560427600 @default.
• W2015972426 cites W1577471567 @default.
• W2015972426 cites W1603566917 @default.
• W2015972426 cites W1606150637 @default.
• W2015972426 cites W1657059619 @default.
• W2015972426 cites W1663294546 @default.
• W2015972426 cites W1746842353 @default.
• W2015972426 cites W1779080088 @default.
• W2015972426 cites W1795729066 @default.
• W2015972426 cites W1814543726 @default.
• W2015972426 cites W1827825926 @default.
• W2015972426 cites W1919785074 @default.
• W2015972426 cites W1927377064 @default.
• W2015972426 cites W1948054437 @default.
• W2015972426 cites W1965385026 @default.
• W2015972426 cites W1982260117 @default.
• W2015972426 cites W2003019382 @default.
• W2015972426 cites W2019212632 @default.
• W2015972426 cites W2020286681 @default.
• W2015972426 cites W2029675011 @default.
• W2015972426 cites W2035997521 @default.
• W2015972426 cites W2040497667 @default.
• W2015972426 cites W2046768926 @default.
• W2015972426 cites W2048480788 @default.
• W2015972426 cites W2051896697 @default.
• W2015972426 cites W2053266196 @default.
• W2015972426 cites W2059617899 @default.
• W2015972426 cites W2066748158 @default.
• W2015972426 cites W2069552780 @default.
• W2015972426 cites W2075398353 @default.
• W2015972426 cites W2087393962 @default.
• W2015972426 cites W2111056094 @default.
• W2015972426 cites W2117113481 @default.
• W2015972426 cites W2120691236 @default.
• W2015972426 cites W2128753750 @default.
• W2015972426 cites W2128984306 @default.
• W2015972426 cites W2134214251 @default.
• W2015972426 cites W2155213376 @default.
• W2015972426 cites W2157966332 @default.
• W2015972426 cites W2170174778 @default.
• W2015972426 cites W2171409124 @default.
• W2015972426 doi "https://doi.org/10.1006/viro.1997.8545" @default.
• W2015972426 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9168889" @default.
• W2015972426 hasPublicationYear "1997" @default.
• W2015972426 type Work @default.
• W2015972426 sameAs 2015972426 @default.
• W2015972426 citedByCount "94" @default.
• W2015972426 countsByYear W20159724262012 @default.
• W2015972426 countsByYear W20159724262013 @default.
• W2015972426 countsByYear W20159724262014 @default.
• W2015972426 countsByYear W20159724262015 @default.
• W2015972426 countsByYear W20159724262016 @default.
• W2015972426 countsByYear W20159724262017 @default.
• W2015972426 countsByYear W20159724262018 @default.
• W2015972426 countsByYear W20159724262019 @default.
• W2015972426 countsByYear W20159724262020 @default.
• W2015972426 countsByYear W20159724262021 @default.
• W2015972426 countsByYear W20159724262022 @default.
• W2015972426 countsByYear W20159724262023 @default.
• W2015972426 crossrefType "journal-article" @default.
• W2015972426 hasAuthorship W2015972426A5005437319 @default.
• W2015972426 hasAuthorship W2015972426A5019410432 @default.
• W2015972426 hasAuthorship W2015972426A5033180835 @default.
• W2015972426 hasAuthorship W2015972426A5061059543 @default.
• W2015972426 hasAuthorship W2015972426A5067304973 @default.
• W2015972426 hasBestOaLocation W20159724261 @default.
• W2015972426 hasConcept C104317684 @default.
• W2015972426 hasConcept C143065580 @default.
• W2015972426 hasConcept C150194340 @default.
• W2015972426 hasConcept C153911025 @default.
• W2015972426 hasConcept C159047783 @default.
• W2015972426 hasConcept C202878990 @default.
• W2015972426 hasConcept C207583985 @default.
• W2015972426 hasConcept C2522874641 @default.
• W2015972426 hasConcept C2776668709 @default.
• W2015972426 hasConcept C2778561560 @default.
• W2015972426 hasConcept C2779630601 @default.
• W2015972426 hasConcept C501734568 @default.
• W2015972426 hasConcept C54355233 @default.
• W2015972426 hasConcept C71829478 @default.
• W2015972426 hasConcept C86803240 @default.
• W2015972426 hasConceptScore W2015972426C104317684 @default.
• W2015972426 hasConceptScore W2015972426C143065580 @default.

• next